The study aimed to evaluate the effect of graphene oxide (GO) nano-filler content in experimental dental adhesive on its degree of conversion (DC), microtensile bond strength (μTBS) and structural reliability, using Fourier transform infrared spectroscopy (FTIR), Micro-Raman spectroscopy and Scanning electron microscopy (SEM). A resin adhesive was fabricated (control adhesive - CA) and fabricated GO nano-particles were added at 0.5% and 2.0% (m/m) to produce adhesives GOA1 and GOA2 respectively. One hundred and two teeth (specimens) were prepared for dentin exposure and conditioned with 36% phosphoric acid. Specimens in each group (n=34) were treated with formulated adhesives (CA, GOA1 & GOA2) and photo-polymerized for 20 s followed by composite build up. Sixty specimens were used for μTBS testing in the adhesive groups (CA, GOA1 & GOA2), with half exposed to thermocycling (TC) whereas the remaining half (n = 10) stored in distilled water. Seven specimens each were assessed using SEM and Micro-Raman spectroscopy, in each adhesive group (n = 7). DC for the adhesives was assessed using FTIR. The means of μTBS and DC were analyzed using ANOVA and post hoc Tukey multiple comparisons test. GO nano-filler content showed significant influence on the adhesive μTBS in comparison to controls (p < 0.01). Ageing (TC) revealed significant reduction in the μTBS, except in GOA2 specimens, which showed comparable outcomes among TC and non-TC specimens (p>0.05). DC was significantly higher in control adhesive [46.8 (3.6)%] compared to GOA2 [37.7 (4.2)%] specimens, however DC was comparable among GOA1 [42.3 (2.9)%] and GOA2 [37.7 (4.2)%] specimens (p > 0.05) respectively. GO exhibited interaction within adhesive and tooth dentin comparable to control adhesive. Increasing GO content showed increase in μTBS of adhesive to dentin, but a decrease in degree of conversion. Under ideal conditions, experimental adhesive with 2% GO content showed acceptable bond strength and DC; and should be further assessed under dynamic conditions to recommend clinical use.

该研究旨在评估实验牙科粘合剂中氧化石墨烯 (GO) 纳米填料含量对其转化度 (DC)、微拉伸粘合强度 ( μ TBS) 和结构可靠性的影响，使用傅里叶变换红外光谱 (FTIR)，显微拉曼光谱和扫描电子显微镜 (SEM)。制造树脂粘合剂（对照粘合剂-CA）并以0.5%和2.0%（m/m）添加制造的GO纳米颗粒以分别制备粘合剂GOA1和GOA2。为牙本质暴露准备了 102 颗牙齿（样本），并用 36% 的磷酸进行了调理。每组（n = 34）的样本用配制的粘合剂（CA、GOA1 和 GOA2）处理并光聚合 20 秒，然后复合材料堆积。60 个样本用于μ粘合剂组（CA、GOA1 和 GOA2）中的 TBS 测试，其中一半暴露于热循环 (TC)，而其余一半 (n = 10) 储存在蒸馏水中。使用 SEM 和显微拉曼光谱对每个粘合剂组 (n = 7) 中的七个样品进行评估。使用 FTIR 评估粘合剂的 DC。μ TBS 和 DC的平均值使用方差分析和事后 Tukey 多重比较检验进行分析。与对照相比，GO 纳米填料含量对粘合剂μ TBS显示出显着影响（p < 0.01）。老化 (TC) 显示μ显着降低TBS，除了 GOA2 标本，其在 TC 和非 TC 标本中显示出可比的结果（p>0.05）。与 GOA2 [37.7 (4.2)%] 样品相比，对照粘合剂 [46.8 (3.6)%] 的 DC 显着更高，但是 GOA1 [42.3 (2.9)%] 和 GOA2 [37.7 (4.2)%] 样品之间的 DC 相当（ p > 0.05) 分别。GO 在粘合剂和牙齿牙本质内表现出与对照粘合剂相当的相互作用。增加 GO 含量表明粘合剂对牙本质的μ TBS增加，但转化程度降低。在理想条件下，GO含量为2%的实验粘合剂表现出可接受的粘合强度和DC；并应在动态条件下进一步评估以推荐临床使用。