In this study, we have developed a paper-based electrochemical biosensor for dopamine (DA) detection. For this purpose, a screen-printed graphene electrode (SPGNE) was printed on a paper substrate, and the electrode surface was modified with PEDOT:PSS/RGO. The fabricated sensor was characterized by analyzing the capacitance, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) measurements. Differential pulse voltammetry (DPV) was employed for the quantitative detection of dopamine. The paper-based SPGNE biosensor developed in this study could detect dopamine in a linear range from 0.0125 to 0.1 mM with a sensitivity of 24.9 μA mM⁻¹. The reliability of the developed biosensor for dopamine detection was assessed by using normal serum samples. The paper-based graphene biosensor proposed in this study could be low-cost and eco-friendly, and its ability to determine the dopamine from real serum samples showed promising results. The developed SPGNE dopamine-sensing biosensor could be potentially applied for the early-stage diagnosis of various neurological diseases/disorders.

在这项研究中，我们开发了一种用于多巴胺 (DA) 检测的纸基电化学生物传感器。为此，将丝网印刷石墨烯电极 (SPGNE) 印刷在纸基材上，并用 PEDOT:PSS/RGO 修饰电极表面。通过分析电容、循环伏安法 (CV) 和电化学阻抗谱 (EIS) 测量来表征制造的传感器。差分脉冲伏安法 (DPV) 用于定量检测多巴胺。本研究开发的纸质 SPGNE 生物传感器可以检测线性范围为 0.0125 至 0.1 mM 的多巴胺，灵敏度为 24.9 μ A mM ^-1. 通过使用正常血清样本评估开发的用于多巴胺检测的生物传感器的可靠性。本研究中提出的纸质石墨烯生物传感器成本低且环保，其从真实血清样本中测定多巴胺的能力显示出令人鼓舞的结果。开发的 SPGNE 多巴胺传感生物传感器有可能应用于各种神经系统疾病/障碍的早期诊断。