Abstract

Purpose

DNA damage is one of the main consequences of exposure to ionizing irradiation (IR). Recent studies indicate that IR can modulate the expression of immune system-related genes. However, the effects of IR on the expression of genes and pathways of the B7-CD28 superfamily remain poorly defined. The aim of this study was to evaluate the modulation of genes and pathways related to the B7-CD28 superfamily in response to IR.

Materials and methods

In this study, we used transcriptome data available from the Gene Expression Omnibus (GEO) database to investigate the modulation of the response of genes and pathways of samples of human peripheral blood irradiated with doses of 150, 300, and 600 cGy. The data were obtained at 6 and 24 h after irradiation. The relationship between genes and pathways was established through the Reactome database. The behavior of these pathways was analyzed using mathematical methods based on relative activity and diversity. Analysis of variance (ANOVA) followed by multiple comparisons tests (Bonferroni and Tamhanes) was used to identify differentially expressed genes. Data on transcriptomes were analyzed through ViaComplex V.1.0 and IBM SPSS Statistics 22.

Results

For the pathways investigated in this study, we observed that the effects produced by these doses significantly modified the behavior of five pathways associated with the immune system. Also, the dose of 300 cGy might trigger signaling for the activation of T cells through the negative regulation (p < .05) of the co-inhibitory PDCD1LG2 gene. Positive regulation caused by 300 cGy (p < .05) of the CD80 receptor was observed by us, which might be related to a stimulatory signal. According to our findings, this dose induced the production of cytokines and genes that are associated with the activation and differentiation of T cells.

Conclusions

Our findings indicate that the irradiation modulated the organization of the biological system, suggesting that 300 cGy is more efficient in activating the immune system.

中文翻译：

---

与 B7-CD28 超家族相关的基因和通路在使用 137Cs 时响应血细胞辐射的表达

摘要

目的

DNA 损伤是暴露于电离辐射 (IR) 的主要后果之一。最近的研究表明，IR 可以调节免疫系统相关基因的表达。然而，IR 对 B7-CD28 超家族基因和途径表达的影响仍然不清楚。本研究的目的是评估响应于 IR 的 B7-CD28 超家族相关基因和途径的调节。

材料和方法

在本研究中，我们使用基因表达综合 (GEO) 数据库中提供的转录组数据来研究人类外周血样本在 150、300 和 600 cGy 剂量下对基因和通路的反应的调节。数据是在辐照后 6 和 24 小时获得的。基因和通路之间的关系是通过Reactome数据库建立的。使用基于相对活性和多样性的数学方法分析这些途径的行为。方差分析（ANOVA）和多重比较检验（Bonferroni 和 Tamhanes）用于鉴定差异表达的基因。通过 ViaComplex V.1.0 和 IBM SPSS Statistics 22 分析转录组数据。

结果

对于本研究中研究的途径，我们观察到这些剂量产生的影响显着改变了与免疫系统相关的五种途径的行为。此外，300 cGy 的剂量可能会通过 共抑制 PDCD1LG2 基因的负调节 ( p < .05)触发 T 细胞活化的信号传导。 我们观察到由 300 cGy ( p < .05) 的 CD80 受体引起的正调节，这可能与刺激信号有关。根据我们的研究结果，该剂量诱导了与 T 细胞活化和分化相关的细胞因子和基因的产生。

结论

我们的研究结果表明，辐射调节了生物系统的组织，表明 300 cGy 在激活免疫系统方面更有效。