当前位置: X-MOL 学术Front. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
The Network of Interactions Between Classical Swine Fever Virus Nonstructural Protein p7 and Host Proteins
Frontiers in Microbiology ( IF 5.2 ) Pub Date : 2020-11-09 , DOI: 10.3389/fmicb.2020.597893
Jindai Fan , Mengru Zhang , Chenchen Liu , Mengjiao Zhu , Zilin Zhang , Keke Wu , Zhaoyao Li , Wenhui Li , Shuangqi Fan , Chunmei Ju , Lin Yi , Hongxing Ding , Mingqiu Zhao , Jinding Chen

Classical swine fever (CSF) is a highly contagious viral disease causing severe economic losses to the swine industry. As viroporins of viruses modulate the cellular ion balance and then take over the cellular machinery, blocking the activity of viroporin or developing viroporin-defective attenuated vaccines offers new approaches to treat or prevent viral infection. Non-structural protein p7 of CSF virus (CSFV) is a viroporin, which was highly involved in CSFV virulence. Deciphering the interaction between p7 and host proteins will aid our understanding of the mechanism of p7-cellular protein interaction affecting CSFV replication. In the present study, seven host cellular proteins including microtubule-associated protein RP/EB family member 1 (MAPRE1), voltage-dependent anion channel 1 (VDAC1), proteasome maturation protein (POMP), protein inhibitor of activated STAT 1 (PIAS1), gametogenetin binding protein 2 (GGNBP2), COP9 signalosome subunit 2 (COPS2), and contactin 1 (CNTN1) were identified as the potential interactive cellular proteins of CSFV p7 by using yeast two-hybrid (Y2H) screening. Plus, the interaction of CSFV p7 with MAPRE1 and VDAC1 was further evaluated by co-immunoprecipitation and GST-pulldown assay. Besides, the p7-cellular protein interaction network was constructed based on these seven host cellular proteins and the STRING database. Enrichment analysis of GO and KEGG indicated that many host proteins in the p7-cellular protein interaction network were mainly related to the ubiquitin-proteasome system, cGMP-PKG signaling pathway, calcium signaling pathway, and JAK-STAT pathway. Overall, this study identified potential interactive cellular proteins of CSFV p7, constructed the p7-cellular protein interaction network, and predicted the potential pathways involved in the interaction between CSFV p7 and host cells.



中文翻译:

猪瘟病毒非结构蛋白p7与宿主蛋白之间的相互作用网络

古典猪瘟(CSF)是一种高度传染性的病毒性疾病,给养猪业造成严重的经济损失。由于病毒的维罗帕林调节细胞离子平衡,然后接管细胞机器,阻断维罗帕林的活性或开发维罗帕林缺陷型减毒疫苗提供了治疗或预防病毒感染的新方法。CSF病毒(CSFV)的非结构蛋白p7是viroporin,与CSFV毒力高度相关。解读p7与宿主蛋白之间的相互作用将有助于我们了解p7-细胞蛋白相互作用影响CSFV复制的机制。在本研究中,七种宿主细胞蛋白包括微管相关蛋白RP / EB家族成员1(MAPRE1),电压依赖性阴离子通道1(VDAC1),蛋白酶体成熟蛋白(POMP),STAT2(PIAS1),配子生成素结合蛋白2(GGNBP2),COP9信号体亚基2(COPS2)和contactin 1(CNTN1)的蛋白抑制剂通过酵母双杂交( Y2H)筛选。另外,还通过共同免疫沉淀和GST下拉试验进一步评估了CSFV p7与MAPRE1和VDAC1的相互作用。此外,基于这七个宿主细胞蛋白和STRING数据库构建了p7-细胞蛋白相互作用网络。GO和KEGG的富集分析表明,p7-细胞蛋白相互作用网络中的许多宿主蛋白主要与泛素-蛋白酶体系统,cGMP-PKG信号通路,钙信号通路和JAK-STAT通路有关。总体,

更新日期:2020-12-01
down
wechat
bug