It was reported that the expression of Toll‐like receptor (TLR) 9 may be related to Th2‐type allergic inflammation including allergic rhinitis (AR). However, little is known about the expression of TLR9 in the basophils in AR. In the present study, the expression of TLR9 was examined by flow cytometry analysis, and the expression of TLR9 mRNA in KU812 was determined by quantitative real‐time PCR. The results showed that the percentage of TLR9⁺CCR3⁺ cells in blood granulocytes increased by 46% in patients with AR, but not in peripheral blood mononuclear cells (PBMCs). Allergens namely Dermatophagoide allergen extract (DAE) and Platanus pollen allergen extract (PPAE) upregulated the expression of TLR9 in CCR3⁺ granulocytes by 76% and 84%, respectively. DAE and PPAE also enhanced the proportions of TLR9⁺CD123⁺HLA‐DR⁻ cells and TLR9⁺CCR3⁺CD123⁺HLA‐DR⁻ cells in granulocytes and PBMCs of patients with AR. In order to investigate the actions of allergens on basophils, KU812 cells were used. It was observed that all KU812 cells expressed TLR9, and the expression intensity of TLR9 in a single KU812 cell was elevated by CpG. IL‐37, IL‐31, IL‐33, Artemisia sieversiana wild allergen extract (ASWAE), DAE, OVA and Der p 1 induced an increase in the expression of TLR9 mRNA and IL‐6 production in KU812 cells. It was shown that the percentage of TLR9‐expressing basophils increased in the blood of ovalbumin (OVA)‐sensitized mice. In conclusion, an increased expression of TLR9 and the production of IL‐6 in basophils implicate that the contribution of basophils to AR is likely via TLR9.

中文翻译：

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过敏性鼻炎患者嗜碱性粒细胞中Toll样受体9的表达上调：变应原的表达增强

据报道，Toll样受体（TLR）9的表达可能与包括过敏性鼻炎（AR）在内的Th2型过敏性炎症有关。然而，关于AR中嗜碱性粒细胞中TLR9的表达知之甚少。在本研究中，通过流式细胞术分析了TLR9的表达，并通过实时定量PCR测定了KU812中TLR9 mRNA的表达。结果显示，AR患者血液粒细胞中TLR9 ⁺ CCR3 ⁺细胞的百分比增加了46％，而外周血单个核细胞（PBMC）没有增加。过敏原，即Dermatophagoide过敏原提取物（DAE）和Platanus花粉过敏原提取物（PPAE）上调了CCR3 ⁺中TLR9的表达。粒细胞分别减少了76％和84％。DAE和PPAE也增强TLR9的比例⁺ CD123 ⁺ HLA-DR ^-的细胞和TLR9 ⁺ CCR3 ⁺ CD123 ⁺ HLA-DR ^-在粒细胞和患者的PBMC AR细胞。为了研究变应原对嗜碱性粒细胞的作用，使用了KU812细胞。观察到所有KU812细胞都表达TLR9，并且在单个KU812细胞中TLR9的表达强度被CpG提高。IL-37，IL-31，IL-33，艾蒿野生变应原提取物（ASWAE），DAE，OVA和Der p 1诱导KU812细胞中TLR9 mRNA表达和IL-6产生增加。结果表明，在卵清蛋白（OVA）致敏小鼠的血液中，表达TLR9的嗜碱性粒细胞百分比增加。总之，嗜碱性粒细胞中TLR9的表达增加和IL-6的产生暗示着嗜碱性粒细胞对AR的贡献可能是通过TLR9引起的。