The kinesin superfamily of proteins (KIFs) are microtubule motor proteins that use the hydrolysis of ATP to power directional movement along microtubules. KIFs induce microtubule depolymerization to regulate the length and dynamics of microtubules in a variety of cell processes and structures, including the mitotic and meiotic spindles and centriole and interphase microtubules. KIF plays a significant role in the transport of organelles, protein complexes and mRNAs. The brown planthopper (Nilaparvata lugens) is a major insect pest in rice paddy fields. Ovarian development is regulated by multiple factors, including endocrine factors. The role of KIFs in brown planthopper ovarian development remains unknown. We found that downregulation of KIF2A significantly compromised the development and eclosion of the brown planthopper, delayed ovarian cell cycle progression, disrupted ovarian development, reduced the expression of MCM genes required for DNA replication and significantly reduced the number of nuclei in the follicles. We also found a significant reduction in Vg mRNA and protein levels. We conclude that downregulation of KIF2A disrupts the cell cycle progression of cells. Alternatively, the ovarian phenotype could be an indirect effect of a compromised trophic cord. In summary, KIF2A regulates ovarian development via modulating cell cycle progression and/or vitollogenin transportation.

中文翻译：

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KIF2A 通过调节细胞周期进程和卵黄蛋白原水平调节卵巢发育

蛋白驱动蛋白超家族 (KIF) 是微管运动蛋白，它利用 ATP 的水解作用为沿微管的定向运动提供动力。KIF 诱导微管解聚以调节各种细胞过程和结构中微管的长度和动力学，包括有丝分裂和减数分裂纺锤体以及中心粒和间期微管。KIF在细胞器、蛋白质复合物和 mRNA 的运输中起着重要作用。褐飞虱（Nilaparvata lugens）是稻田的主要害虫。卵巢发育受多种因素调节，包括内分泌因素。KIFs 在褐飞虱卵巢发育中的作用仍然未知。我们发现KIF2A 的下调显着影响褐飞虱的发育和羽化，延迟卵巢细胞周期进程，破坏卵巢发育，降低DNA 复制所需的MCM基因的表达，并显着减少卵泡中的细胞核数量。我们还发现Vg mRNA 和蛋白质水平显着降低。我们得出结论，KIF2A 的下调会破坏细胞的细胞周期进程。或者，卵巢表型可能是营养索受损的间接影响。总之，KIF2A通过调节细胞周期进程和/或卵黄蛋白原运输来调节卵巢发育。