Transmembrane anion transport is an important biological process in maintaining cellular functions. Thus, synthetic anion transporters are widely developed for their biological applications. Imidazolinium was introduced as anion recognition site to a multiblock amphiphilic structure that consists of octa(ethylene glycol) and aromatic units. Ion transport assay using halide‐sensitive lucigenin and pH‐sensitive 8‐hydroxypyrene‐1,3,6‐trisulfonate (HPTS) revealed that imidazolinium‐based multiblock amphiphile (IMA) transports anions and showed high selectivity for nitrate, which plays crucial roles in many biological events. Temperature‐dependent ion transport assay using 1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphocholine (DPPC) indicated that IMA works as a mobile carrier. ¹H NMR titration experiments indicated that the C2 proton of the imidazolinium ring recognizes anions via a (C−H)⁺⋅⋅⋅X⁻ hydrogen bond. Furthermore, all‐atom molecular dynamics simulations revealed a dynamic feature of IMA within the membranes during ion transportation.

中文翻译：

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基于咪唑啉鎓的多嵌段两亲物作为跨膜阴离子转运蛋白

跨膜阴离子转运是维持细胞功能的重要生物学过程。因此，合成阴离子转运蛋白已被广泛开发用于其生物学应用。将咪唑啉鎓作为阴离子识别位点引入由八（乙二醇）和芳族单元组成的多嵌段两亲结构。使用卤化物敏感的光泽精和pH敏感的8-羟基py 1,3,6-三磺酸盐（HPTS）进行离子迁移测定表明，基于咪唑啉鎓的多嵌段两亲物（IMA）可以迁移阴离子并显示出对硝酸盐的高选择性，这在硝酸盐中起关键作用许多生物学事件。使用1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）的温度依赖性离子迁移测定表明IMA充当移动运营商。^1个1 H NMR滴定实验表明，咪唑啉环的C2质子识别经由（C-H）阴离子⁺ ⋅⋅⋅X ^-氢键。此外，全原子分子动力学模拟揭示了离子传输过程中膜内IMA的动力学特征。