当前位置: X-MOL 学术Angew. Chem. Int. Ed. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Hierarchical Self‐assembly of Discrete Metal–Organic Cages into Supramolecular Nanoparticles for Intracellular Protein Delivery
Angewandte Chemie International Edition ( IF 16.6 ) Pub Date : 2020-11-27 , DOI: 10.1002/anie.202013904
Ji Liu 1, 2 , Tianli Luo 1, 2 , Yifei Xue 1, 2 , Lanqun Mao 1, 2 , Peter J Stang 3 , Ming Wang 1, 2
Affiliation  

Hierarchical self‐assembly (HAS) is a powerful approach to create supramolecular nanostructures for biomedical applications. This potency, however, is generally challenged by the difficulty of controlling the HAS of biomacromolecules and the functionality of resulted HAS nanostructures. Herein, we report a modular approach for controlling the HAS of discrete metal–organic cages (MOC) into supramolecular nanoparticles, and its potential for intracellular protein delivery and cell‐fate specification. The hierarchical coordination‐driven self‐assembly of adamantane‐functionalized M12L24 MOC (Ada‐MOC) and the host–guest interaction of Ada‐MOC with β‐cyclodextrin‐conjugated polyethylenimine (PEI‐βCD) afford supramolecular nanoparticles in a controllable manner. HAS maintains high efficiency and orthogonality in the presence of protein, enabling the encapsulation of protein into the nanoparticles for intracellular protein delivery for therapeutic application and CRISPR/Cas9 genome editing.

中文翻译:

离散金属-有机笼的层级自组装成超分子纳米颗粒用于细胞内蛋白递送。

分层自组装(HAS)是一种强大的方法,可以为生物医学应用创建超分子纳米结构。但是,这种效力通常受到控制生物大分子HAS的困难以及所得HAS纳米结构功能的挑战。在本文中,我们报告了一种模块化方法,用于控制将离散的金属-有机笼(MOC)的HAS转化为超分子纳米颗粒,以及其在细胞内蛋白递送和细胞命运规范中的潜力。金刚烷官能化的M 12 L 24的分层协调驱动的自组装MOC(Ada-MOC)和Ada-MOC与β-环糊精偶联的聚乙烯亚胺(PEI-βCD)的宿主-客体相互作用可控地提供超分子纳米颗粒。HAS在蛋白质存在的情况下保持高效率和正交性,从而能够将蛋白质封装到纳米颗粒中以进行细胞内蛋白质递送,从而进行治疗应用和CRISPR / Cas9基因组编辑。
更新日期:2020-11-27
down
wechat
bug