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Ultrasound Microbubble-Mediated VHL Regulates the Biological Behavior of Ovarian Cancer Cells
Ultrasound in Medicine & Biology ( IF 2.9 ) Pub Date : 2020-11-28 , DOI: 10.1016/j.ultrasmedbio.2020.11.001
Cong Li 1 , Suling Hu 2 , Yan Yue 3
Affiliation  

According to the literature, the von Hippel–Lindau (VHL) gene has a certain correlation with ovarian cancer. In this study, we investigated the effect and mechanism of ultrasound microbubble-mediated VHL on the biological function of ovarian cancer cells. Non-targeting lipid microbubbles and targeted lipid microbubbles were prepared. OVCAR-3 cells were treated with VHL mediated by ultrasound and microbubbles alone or together. Expressions of VHL, Akt, epithelial–mesenchymal-transition-related proteins and apoptosis-related proteins were detected by Western blot and quantitative real-time polymerase chain reaction as needed. The effect of ultrasound microbubble-mediated VHL on the proliferation, apoptosis, cell cycle, migration and invasion of OVCAR-3 cells was examined by Cell Counting Kit-8, flow cytometry, wound-healing assay and Transwell. Compared with other treatment methods, ultrasound microbubble mediation enhanced VHL expression in OVCAR-3 cells. Overexpression of liposome-mediated VHL inhibited the proliferation and migration; caused cell-cycle arrest; promoted apoptosis: downregulated the expressions of MMP2, MMP9, E-cadherin, Akt and Bcl-2; and upregulated the expressions of VHL and BCL2-associated X protein (BAX) in OVCAR-3 cells. The effect of microbubble-treated VHL was similar to liposome-mediated regulation, while ultrasound treatment enhanced the effect of VHL on OVCAR-3 cells. More interestingly, ultrasound microbubble-mediated VHL had the most obvious regulatory effect on OVCAR-3 cells. Ultrasound microbubble technology increases the transfection efficiency of VHL into OVCAR-3 cells and enhances the effect of VHL gene on the biological function of OVCAR-3 cells.



中文翻译:

超声微泡介导的 VHL 调节卵巢癌细胞的生物学行为

据文献报道,von Hippel-Lindau(VHL)基因与卵巢癌有一定的相关性。在这项研究中,我们研究了超声微泡介导的 VHL 对卵巢癌细胞生物学功能的影响和机制。制备了非靶向脂质微泡和靶向脂质微泡。OVCAR-3 细胞用由超声和微泡单独或一起介导的 VHL 处理。根据需要通过蛋白质印迹和定量实时聚合酶链反应检测 VHL、Akt、上皮间充质转化相关蛋白和细胞凋亡相关蛋白的表达。通过Cell Counting Kit-8、流式细胞术、伤口愈合试验和Transwell检测超声微泡介导的VHL对OVCAR-3细胞增殖、凋亡、细胞周期、迁移和侵袭的影响。与其他治疗方法相比,超声微泡介导增强了 OVCAR-3 细胞中 VHL 的表达。脂质体介导的VHL过表达抑制增殖和迁移;导致细胞周期停滞;促进细胞凋亡:下调MMP2、MMP9、E-cadherin、Akt和Bcl-2的表达;并上调OVCAR-3细胞中VHL和BCL2相关X蛋白(BAX)的表达。微泡处理的 VHL 的作用类似于脂质体介导的调节,而超声处理增强了 VHL 对 OVCAR-3 细胞的作用。更有趣的是,超声微泡介导的 VHL 对 OVCAR-3 细胞的调节作用最为明显。超声微泡技术提高了VHL转染OVCAR-3细胞的效率,增强了VHL基因对OVCAR-3细胞生物学功能的影响。

更新日期:2021-01-15
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