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Composition and functional comparison of vetiver root endophytic microbiota originating from different geographic locations that show antagonistic activity towards Fusarium graminearum
Microbiological Research ( IF 6.7 ) Pub Date : 2020-11-28 , DOI: 10.1016/j.micres.2020.126650
Yuka Munakata 1 , Carole Gavira 2 , Julie Genestier 1 , Frédéric Bourgaud 2 , Alain Hehn 1 , Sophie Slezack-Deschaumes 1
Affiliation  

Given the current trend towards reducing the use of chemical controls in agriculture, microbial resources such as plant endophytes are being intensively investigated for traits that are conducive to plant protection. Among the various important target pathogens, Fusarium graminearum is a fungal pathogen of cereal crops that is responsible for severe yield losses and mycotoxin contamination in grains. In the present study, we investigated the bacterial endophytic communities from vetiver (Chrysopogon zizanioides (L.) Roberty) roots originating from 5 different geographic locations across Europe and Africa. This study relies on a global 16S metabarcoding approach and the isolation/functional characterization of bacterial isolates. The results we obtained showed that geographical location is a factor that influences the composition and relative abundance of root endophyte communities in vetiver. Three hundred eighty-one bacterial endophytes were isolated and assessed for their in vitro antagonistic activities towards F. graminearum mycelium growth. In total, 46% of the isolates showed at least 50% inhibitory activity against F. graminearum. The taxonomic identification of the bioactive isolates revealed that the composition of these functional culturable endophytic communities was influenced by the geographic origins of the roots. The selected communities consisted of 15 genera. Some endophytes in Bacillus, Janthinobacterium, Kosakonia, Microbacterium, Pseudomonas, and Serratia showed strong growth inhibition activity (≥ 70%) against F. graminearum and could be candidates for further development as biocontrol agents.



中文翻译:

不同地理位置对香蒲镰刀菌具有拮抗作用的香根根内生菌群的组成和功能比较

鉴于当前减少农业中使用化学控制手段的趋势,人们正在对诸如植物内生菌之类的微生物资源进行有益于植物保护的性状研究。在各种重要的目标病原体中,镰孢(Fusarium graminearum)是谷类作物的真菌病原体,导致谷物的严重减产和霉菌毒素污染。在本研究中,我们调查了香根草(Chrysopogon zizanioides)的细菌内生群落(L.)Roberty)起源于欧洲和非洲的5个不同地理位置。这项研究依赖于全球16S元条形码方法和细菌分离株的分离/功能表征。我们获得的结果表明,地理位置是影响香根草根内生植物群落组成和相对丰度的因素。分离了381个细菌内生菌并评估了它们对镰刀菌菌丝体生长的体外拮抗活性。总共有46%的分离株对禾谷镰孢具有至少50%的抑制活性。生物活性分离物的分类学鉴定表明,这些功能性可培养内生群落的组成受根的地理起源影响。选定的社区由15个属组成。在某些内生菌芽孢杆菌JanthinobacteriumKosakonia微杆菌假单胞菌,和沙雷氏菌属显示出对强的生长抑制活性(≥70%)禾谷镰刀菌,并且可以是用于进一步开发作为生物控制剂的候选者。

更新日期:2020-12-01
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