This work describes an ultrasound-assisted dispersive solid-phase extraction method combined with reversed-phase high-performance liquid chromatography-photodiode array detection (UADSPE-RPLC-PDA) for the determination of nine common biogenic amines (BAs) in canned seafood. The pretreatment extraction solvent, ultrasonic treatment duration, and derivatization conditions were optimized. The method was validated on the basis of the limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy and successfully applied to analyze four canned fish, two canned shrimp and four canned shellfish samples. LODs of 0.08–0.25 mg kg⁻¹ were achieved, and the correlation coefficient of determination was 0.9994–0.9997. The method had high precision and accuracy, with relative standard deviations (RSDs) and recoveries of 0.44 to 6.83% and 72.57 to 99.74%, respectively, suggesting the effectiveness of ultrasound-assisted extraction at increasing the solubility of the target analytes in the solvent system and the feasibility of UADSPE-RPLC-PDA for determining trace BAs in canned seafood.

这项工作描述了超声辅助分散固相萃取方法与反相高效液相色谱-光电二极管阵列检测（UADSPE-RPLC-PDA）的组合，用于测定罐头海鲜中的九种常见生物胺。优化了预处理萃取溶剂，超声处理时间和衍生化条件。该方法在检出限（LOD），定量限（LOQ），精度和准确性的基础上进行了验证，并成功地用于分析4种罐头鱼，2种虾罐头和4种贝类罐头样品。LOs为0.08–0.25 mg kg ^-1的测定相关系数为0.9994–0.9997。该方法具有较高的精密度和准确性，相对标准偏差（RSD）和回收率分别为0.44至6.83％和72.57至99.74％，这表明超声辅助萃取可提高目标分析物在溶剂系统中的溶解度。 UADSPE-RPLC-PDA测定罐装海鲜中痕量BA的可行性。