Studies of nuclear architecture using chromosome conformation capture methods have provided a detailed view of how chromatin folds in the 3D nuclear space. New variants of this technology now afford unprecedented resolution and allow the identification of ever smaller folding domains that offer new insights into the mechanisms by which this organization is established and maintained. Here we review recent results in this rapidly evolving field with an emphasis on CTCF function, with the goal of gaining a mechanistic understanding of the principles by which chromatin is folded in the eukaryotic nucleus.

中文翻译：

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CTCF 对 3D 染色质组织的调节

使用染色体构象捕获方法对核结构的研究提供了染色质如何在 3D 核空间中折叠的详细视图。这项技术的新变体现在提供了前所未有的分辨率，并允许识别更小的折叠域，从而为该组织的建立和维护机制提供新的见解。在这里，我们回顾这个快速发展的领域的最新结果，重点是 CTCF 功能，目的是获得对染色质在真核细胞核中折叠原理的机械理解。