Circadian clock genes are found in almost every cell that has a nucleus; they regulate the rhythmic nature of all processes that are cyclical. Among the genes controlled by the circadian clock, there are numerous factors that regulate key processes in the functioning of the cell. Disturbances in the functioning of the circadian clock are associated with numerous disorders. A recent study has shown the key role of H₂S in regulating circadian rhythm. In this study, we investigated the in vitro effect of pharmacological inhibition of cystathionine-β-synthase (CBS) and/or cystathionine-γ-lyase (CSE) on the circadian dynamics of Per2 expression in serum-shocked NIH-3T3 cells. Alternatively, Cbs and Cse were knocked down by transfection with siRNA. The 48-h treatment of serum-shocked NIH-3T3 cells with 1 mM dl-propargylglycine (PAG), a specific CSE inhibitor, significantly decreased the amplitude and baseline expression of Per2. During exposure to an effective CBS and CSE inhibitor (aminooxyacetic acid [AOAA]), the amplitude of oscillation and baseline expression of Per2 significantly increased. Incubation of NIH-3T3 cells with both inhibitors also significantly increased the amplitude and baseline expression of Per2 messenger RNA (mRNA). siCbs or siCse knockdowan significantly reduced the baseline and amplitude of oscillation of Per2. In conclusion, we showed that CBS/CSE/H₂S pathway participates in the regulation of the circadian clock system. PAG and AOAA, change the general expression and dynamics of Per2 genes, but the increase of amplitude and overall Per2 mRNA level due to exposure to AOAA is probably caused by factors other than CBS and CSE activity.

几乎在每个具有核的细胞中都存在昼夜节律基因。它们调节所有周期性过程的节奏性。在生物钟控制的基因中，有许多因素可调节细胞功能的关键过程。昼夜节律时钟的功能紊乱与许多疾病有关。最近的一项研究表明H ₂ S在调节昼夜节律中起关键作用。在这项研究中，我们研究了在体外的胱硫醚药理学抑制的效果β合酶（CBS）和/或胱硫醚γ上的昼夜动态裂解酶（CSE）Per2基因表达在无血清震惊NIH-3T3细胞。或者，Cbs通过用siRNA转染来敲除Cse和Cse。用一种特异的CSE抑制剂1 mM dl-炔丙基甘氨酸（PAG）处理血清震惊的NIH-3T3细胞48小时，可显着降低Per2的振幅和基线表达。在暴露于有效的CBS和CSE抑制剂（氨基氧乙酸[AOAA]）期间，Per2的振荡幅度和基线表达显着增加。用两种抑制剂孵育NIH-3T3细胞也显着增加了Per2 Messenger RNA（mRNA）的幅度和基线表达。siCbs或siCse基因敲除显着降低了Per2的基线和振幅。总之，我们表明CBS / CSE / H ₂ S通路参与了生物钟系统的调节。PAG和AOAA会改变Per2基因的一般表达和动态，但是由于暴露于AOAA而引起的振幅和整体Per2 mRNA水平的增加可能是由CBS和CSE活性以外的因素引起的。