Surface functionalization of lipid-based nanocarriers (LBNCs) with targeting ligands has attracted huge interest in the field of nanomedicines for their ability to overcome some physiological barriers and their potential to deliver an active molecule to a specific target without causing damage to healthy tissues. The principal objective of this review is to summarize the present knowledge on LBNC decoration used for biomedical applications, with an emphasis on the ligands used, the functionalization approaches, and the purification methods after ligand corona formation. The most potent experimental techniques for the LBNC surface characterization are described. The potential of promising methods such as nuclear magnetic resonance spectroscopy and isothermal titration calorimetry to characterize ligand surface corona is also outlined.

中文翻译：

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功能化、纯化和表征表面修饰脂质纳米载体的相关物理化学方法

具有靶向配体的基于脂质的纳米载体 (LBNC) 的表面功能化在纳米医学领域引起了极大的兴趣，因为它们能够克服一些生理障碍，并且具有将活性分子传递到特定目标而不会对健康组织造成损害的潜力。本综述的主要目的是总结用于生物医学应用的 LBNC 装饰的现有知识，重点是所用的配体、功能化方法和配体电晕形成后的纯化方法。描述了 LBNC 表面表征的最有效的实验技术。还概述了诸如核磁共振光谱和等温滴定量热法等有前景的方法来表征配体表面电晕的潜力。