Background. Aedes aegypti is a major vector of arboviruses that may be controlled on an area-wide basis, using novel approaches such as Sterile Insect Technique (SIT) and Incompatible Insect Technique (IIT). Larval diet is a critical factor to be considered in mass rearing of Aedes mosquitoes for SIT and IIT programs. Therefore, the current study is aimed at evaluating the effects of two novel diets developed from dry fish powder on the growth and development of immature stages and adult fitness-related characteristics of Ae. aegypti in Sri Lanka. Method. Three batches of the first instar Ae. aegypti larva, each containing 250 larvae, were exposed to three different larval diets as standard dry fish powder (D₁), dry fish powder meal and brewer’s yeast (D₂), and International Atomic Energy Agency- (IAEA-) recommended diet (D₃), separately. Morphometric and developmental parameters of the 4^th instar larvae, pupae, and adult mosquitoes reared under different dietary treatments were measured. The entire experimental setup was replicated thrice. A General Linear Model (GLM) in the form of two-way ANOVA was used for the statistical analysis. Results. Significant diet-based variations were observed in the head length, head width, thoracic length, thoracic width, abdominal length, abdominal width, and total length (; ) of Ae. aegypti larvae. The highest pupation success and the larval size were observed from the larvae fed the D₂ diet, while the lowest was reported from D₁. All adult morphometric parameters of adult male and female Ae. aegypti mosquitoes also denoted significant dietary variations, reporting the best-sized adults from the D₂ diet (; ). Further, significantly higher fecundity and male longevity were also shown by the adult Ae. aegypti (; ) mosquitoes reared under diet D₂. Conclusion. Based on all the growth and developmental parameters, the D₂ diet tends to perform similar to the IAEA-recommended diet in mass rearing of Ae. aegypti mosquitoes, while being more inexpensive. Therefore, larval diet D₂ could be suggested as the ideal diet for mass rearing of Ae. aegypti for IIT and SIT-based vector control in Sri Lanka.

中文翻译：

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开发用于大规模饲养埃及伊蚊的替代低成本幼虫饮食

背景。埃及伊蚊是虫媒病毒的主要载体，可使用昆虫不育技术 (SIT) 和昆虫不相容技术 (IIT) 等新方法在大范围内进行控制。幼虫饮食是为 SIT 和 IIT 计划大规模饲养伊蚊的关键因素。因此，本研究旨在评估由干鱼粉开发的两种新型日粮对Ae 未成熟期生长发育和成年体适能相关特征的影响。斯里兰卡的埃及。方法。三批一龄Ae。埃及人将每只含有 250 只幼虫的幼虫暴露于三种不同的幼虫日粮，即标准干鱼粉 (D ₁ )、干鱼粉粉和啤酒酵母 (D ₂ ) 以及国际原子能机构 (IAEA-) 推荐的日粮 (D ₃ )，分别。^{测量了在不同饮食处理下饲养的 4龄}幼虫、蛹和成蚊的形态和发育参数。整个实验装置重复三次。采用双向 ANOVA 形式的一般线性模型 (GLM) 进行统计分析。结果. 在头长、头宽、胸长、胸宽、腹长、腹宽和总长方面观察到显着的饮食差异。; )的Ae。埃及伊蚊幼虫。_{从喂食 D 2}饮食的幼虫观察到最高的化蛹成功率和幼虫大小，而从 D ₁报告的最低。成年男性和女性Ae的所有成人形态测量参数埃及蚊子也表现出显着的饮食变化，报告了 D ₂饮食中体型最大的成年人（; ）。此外，成年Ae也显示出显着更高的繁殖力和男性寿命埃及(; )_{在饮食 D 2}下饲养的蚊子。结论。根据所有生长和发育参数，D ₂日粮在大规模饲养Ae 方面的表现往往与 IAEA 推荐的日粮相似。埃及蚊子，虽然更便宜。因此，幼虫日粮 D ₂可被认为是大规模饲养Ae 的理想日粮。aegypti用于斯里兰卡的 IIT 和基于 SIT 的病媒控制。