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Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects
Frontiers in Genetics ( IF 3.7 ) Pub Date : 2020-11-04 , DOI: 10.3389/fgene.2020.596947
Elena Yushkova 1
Affiliation  

The study of the genetic basis of the manifestation of radiation-induced effects and their transgenerational inheritance makes it possible to identify the mechanisms of adaptation and possible effective strategies for the survival of organisms in response to chronic radioactive stress. One persistent hypothesis is that the activation of certain genes involved in cellular defense is a specific response of the cell to irradiation. There is also data indicating the important role of transposable elements in the formation of radiosensitivity/radioresistance of biological systems. In this work, we studied the interaction of the systems of hobo transposon activity and DNA repair in the cell under conditions of chronic low-dose irradiation and its participation in the inheritance of radiation-induced transgenerational instability in Drosophila. Our results showed a significant increase of sterility and locus-specific mutability, a decrease of survival, fertility and genome stability (an increase the frequency of dominant lethal mutations and DNA damage) in non-irradiated F1/F2 offspring of irradiated parents with dysfunction of the mus304 gene which is responsible for excision and post-replicative recombination repair and repair of double-stranded DNA breaks. The combined action of dysfunction of the mus309 gene and transpositional activity of hobo elements also led to the transgenerational effects of irradiation but only in the F1 offspring. Dysfunction of the genes of other DNA repair systems (mus101 and mus210) showed no visible effects inherited from irradiated parents subjected to hobo transpositions. The mei-41 gene showed specificity in this type of interaction, which consists in its higher efficiency in sensing events induced by transpositional activity rather than irradiation.



中文翻译:

DNA修复基因和辐射诱导转座子激活系统参与跨代效应的形成

对辐射引起的效应表现的遗传基础及其跨代遗传的研究,使得确定生物体响应慢性放射性应激的适应机制和可能的有效生存策略成为可能。一个持久的假设是,某些参与细胞防御的基因的激活是细胞对辐射的一种特定反应。还有数据表明转座因子在生物系统放射敏感性/放射抗性形成中的重要作用。在这项工作中,我们研究了系统的相互作用流浪汉慢性低剂量辐照条件下细胞内转座子活性和DNA修复及其参与辐射诱导的跨代不稳定性的遗传果蝇。我们的结果表明,经过辐射的父母的未辐射的 F 1 /F 2后代的不育性和位点特异性突变性显着增加,存活率、生育力和基因组稳定性下降(显性致死突变和 DNA 损伤的频率增加)的功能障碍mus304负责切除和复制后重组修复以及双链DNA断裂修复的基因。功能障碍的联合作用穆斯309基因和转座活性流浪汉元素也会导致辐射的跨代效应,但仅限于 F 1后代。其他 DNA 修复系统的基因功能障碍(穆斯101穆斯210)没有表现出从受辐射的父母那里继承的明显影响流浪汉换位。这梅41基因在这种类型的相互作用中表现出特异性,这在于它在感应转座活动而不是辐射诱导的事件方面效率更高。

更新日期:2020-11-27
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