In this paper, we developed a spheroid culture device that can trap a spheroid in the trapping site sandwiched by two extracellular matrix gels located at the upper and lower side of the spheroid. This device can form different biochemical gradients by applying target biochemicals separately in upper and lower channels, allowing us to study the angiogenic sprouting under various biochemical gradients in different directions. In the experiments, we confirmed the trapping of the spheroids and demonstrate the investigation on the direction and extent of angiogenic sprouts under unidirectional or bidirectional biochemical gradients. We believe our device can contribute to understanding the pathophysiological phenomena driven by chemical gradients, such as tissue development and tumor angiogenesis.

中文翻译：

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双向生化梯度下用于微血管分析的微流控装置

在本文中，我们开发了一种球状体培养装置，该装置可以将球状体捕获在由两个位于球体上下两侧的细胞外基质凝胶夹在中间的捕获位点中。通过在上下通道中分别应用目标生化试剂，该设备可以形成不同的生化梯度，从而使我们能够研究不同方向上各种生化梯度下的血管新生。在实验中，我们证实了类球体的捕获，并证明了在单向或双向生化梯度下对血管新生芽的方向和范围的研究。我们相信我们的设备可以帮助理解由化学梯度驱动的病理生理现象，例如组织发育和肿瘤血管生成。