当前位置: X-MOL 学术Sci. Rep. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Salidroside regulates inflammatory pathway of alveolar macrophages by influencing the secretion of miRNA-146a exosomes by lung epithelial cells
Scientific Reports ( IF 4.6 ) Pub Date : 2020-11-27 , DOI: 10.1038/s41598-020-77448-6
Lanzhi Zheng , Jianming Su , Zhuoyi Zhang , Lu Jiang , Jinling Wei , Xiaoyang Xu , Shumin Lv

The purpose of this study was to explore the investigative mechanism of salidroside (SAL) on LPS-induced acute lung injury (ALI)/acute respiratory distress syndrome (ARDS). The exosomes from RLE-6TN are extracted and identified by transmission electron microscopy, particle size analysis and protein marker detection, and co-cultured with NR8383 cells. The ALI/ARDS model of SD rats was established by LPS (10 mg/kg) intratracheal instillation. Following a four-hour intratracheal instillation of LPS, 50 μl of RLE-6TN exosomes were injected through the tail vein. After that, SAL and miR-146a antagomir were injected into the tail vein for 72 h, respectively. As the changes of HE stain, body weight and ALI score are observed. The expression of miR-146a, TLR4, NF-kB, IRAK1, TRAF6 and their related proteins were detected by RT-PCR and Western blot, respectively. TNF-α, IL-6, IL-8 and IL-1 β inflammatory factors were detected by ELISA. The expression of miR-146a, NF-kB, IRAK, TRAF6 and related inflammatory factors in LPS-induced NR8383 was significantly higher than that in the control group, while SAL has greatly reduced the expression of TLR4 mediated NF-kB inflammatory pathway and related inflammatory factors. SAL can significantly improve the LPS-induced lung morphological abnormalities, slowed down the rate of weight loss in rats, and reducing the ALI score. The expression trend of NF-kB, IRAK, TRAF6 and related inflammatory factors in rats’ lung tissues was consistent with that in NR8383 cells. SAL has a protective effect on ALI/ARDS caused by sepsis, which is likely to be developed to a potential treatment for the disease. To sum up, this study provides a new theoretical basis for the treatment of ALI/ARDS with SAL.



中文翻译:

红景天苷通过影响肺上皮细胞分泌miRNA-146a外泌体来调节肺泡巨噬细胞的炎症途径

这项研究的目的是探讨红景天苷(SAL)对LPS诱发的急性肺损伤(ALI)/急性呼吸窘迫综合征(ARDS)的研究机制。提取RLE-6TN的外来体并通过透射电子显微镜,粒度分析和蛋白质标记物检测进行鉴定,并与NR8383细胞共培养。通过LPS(10 mg / kg)气管内滴注建立SD大鼠的ALI / ARDS模型。气管内滴注LPS 4小时后,通过尾静脉注射50μlRLE-6TN外泌体。之后,将SAL和miR-146a antagomir分别注入尾静脉72 h。随着HE染色的变化,观察到体重和ALI得分。用RT-PCR和Western blot检测miR-146a,TLR4,NF-kB,IRAK1,TRAF6及其相关蛋白的表达,分别。通过ELISA检测TNF-α,IL-6,IL-8和IL-1β炎性因子。LPS诱导的NR8383中miR-146a,NF-kB,IRAK,TRAF6及相关炎症因子的表达明显高于对照组,而SAL大大降低了TLR4介导的NF-kB炎症途径及相关因子的表达。炎性因子。SAL可以显着改善LPS诱导的肺形态异常,减慢大鼠体重减轻的速度,并降低ALI评分。大鼠肺组织中NF-κB,IRAK,TRAF6及相关炎症因子的表达趋势与NR8383细胞一致。SAL对败血症引起的ALI / ARDS具有保护作用,很可能已发展成为该疾病的潜在治疗方法。总结一下,

更新日期:2020-11-27
down
wechat
bug