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A relaxin‐like gonad‐stimulating peptide identified from the starfish Astropecten scoparius
Molecular Reproduction and Development ( IF 2.5 ) Pub Date : 2020-11-26 , DOI: 10.1002/mrd.23444
Masatoshi Mita 1 , Tomohiro Osugi 2 , Shin Matsubara 2 , Tsuyoshi Kawada 2 , Honoo Satake 2 , Hidekazu Katayama 3
Affiliation  

A relaxin‐like gonad‐stimulating peptide (RGP) in starfish was the first identified invertebrate gonadotropin responsible for final gamete maturation. An RGP ortholog was newly identified from Astropecten scoparius of the order Paxillosida. The A. scoparius RGP (AscRGP) precursor is encoded by a 354 base pair open reading frame and is a 118 amino acid (aa) protein consisting of a signal peptide (26 aa), B‐chain (21 aa), C‐peptide (47 aa), and A‐chain (24 aa). There are three putative processing sites (Lys‐Arg) between the B‐chain and C‐peptide, between the C‐peptide and A‐chain, and within the C‐peptide. This structural organization revealed that the mature AscRGP is composed of A‐ and B‐chains with two interchain disulfide bonds and one intrachain disulfide bond. The C‐terminal residues of the B‐chain are Gln‐Gly‐Arg, which is a potential substrate for formation of an amidated C‐terminal Gln residue. Non‐amidated (AscRGP‐GR) and amidated (AscRGP‐NH2) peptides were chemically synthesized and their effect on gamete shedding activity was examined using A. scoparius ovaries. Both AscRGP‐GR and AscRGP‐NH2 induced oocyte maturation and ovulation in similar dose‐dependent manners. This is the first report on a C‐terminally amidated functional RGP. Collectively, these results suggest that AscRGP‐GR and AscRGP‐NH2 act as a natural gonadotropic hormone in A. scoparius.

中文翻译:

从海星 Astropecten scoparius 中鉴定出的类似松弛素的性腺刺激肽

海星中的松弛素样性腺刺激肽 (RGP) 是第一个确定的负责最终配子成熟的无脊椎动物促性腺激素。从PaxillosidaAstropecten scoparius中新发现了一个 RGP 直系同源物。该A. scopariusRGP (AscRGP) 前体由 354 碱基对开放阅读框编码,是一种 118 个氨基酸 (aa) 的蛋白质,由信号肽 (26 aa)、B 链 (21 aa)、C 肽 (47 aa) 组成, 和 A 链 (24 aa)。在 B 链和 C 肽之间、C 肽和 A 链之间以及 C 肽内有三个假定的加工位点 (Lys-Arg)。这种结构组织表明成熟的 AscRGP 由具有两个链间二硫键和一个链内二硫键的 A 链和 B 链组成。B 链的 C 端残基是 Gln-Gly-Arg,它是形成酰胺化 C 端 Gln 残基的潜在底物。化学合成了非酰胺化 (AscRGP-GR) 和酰胺化 (AscRGP-NH 2 ) 肽,并使用以下方法检查了它们对配子脱落活性的影响A. scoparius卵巢。AscRGP-GR 和 AscRGP-NH 2均以类似的剂量依赖性方式诱导卵母细胞成熟和排卵。这是关于 C 端酰胺化功能性 RGP 的第一份报告。总的来说,这些结果表明 AscRGP-GR 和 AscRGP-NH 2A. scoparius 中充当天然促性腺激素。
更新日期:2021-01-28
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