Edwardsiella piscicida (E. piscicida) is an important fish pathogen. However, the mechanism of Glu6P transport regulatory protein UhpA how to affect the virulence gene expression in E. piscicida is still unclear. The results in this study showed that the metabolism‐related gene expression of cysteine synthase (orf 1134) and sulphate transporter (ychM) in the uhpA mutant strain ΔuhpA was 0.76‐fold and 0.68‐fold lower than the ones in the wild strains (p < .05). The gene expression of ethA and ethB in the ΔuhpA strain was 0.80‐fold and 0.72‐fold lower than the ones in the wild strains (p < .05). However, the gene expression of fliC and flgN in the ΔuhpA was 1.51‐fold and 1.21‐fold higher than the ones in the wild strains (p < .05). The gene expression of T3SS (esrB and esrC) and T6SS (evpB and evpC) in the ΔuhpA was 1.27‐fold, 1.13‐fold, 1.28‐fold and 1.23‐fold higher than the ones in the wild strains (p < .05). This suggested that the uhpA gene could regulate the key virulence gene expression, and the uhpA gene was associated with the pathogenicity of E. piscicida in fish.

中文翻译：

---

UhpA在调节爱德华氏菌中毒力基因表达中的作用

爱德华piscicida（E. piscicida）是一个重要的鱼类病原体。然而，Glu6P转运调节蛋白UhpA如何影响在E. piscicida中的毒力基因表达的机制仍不清楚。这项研究的结果表明，uhp A突变株ΔuhpA中半胱氨酸合酶（orf 1134）和硫酸盐转运蛋白（ych M）的代谢相关基因表达比野生动物低0.76倍和0.68倍应变（p  <.05）。ETHA和ETHB在Δ基因表达UHP应变为0.80倍及0.72倍的野生菌株的那些较低（p <.05）。然而，基因表达的fliC和flgN在Δ uhpA为1.51倍和比野生株的那些（1.21倍高的p  <0.05）。T3SS（基因表达ESRB和ESRC）和T6SS（evpB和evpC在Δ）uhpA为1.27倍，1.13倍，1.28倍和1.23倍的野生株的那些（较高p  <0.05 ）。这表明uhp A基因可以调节关键毒力基因的表达，而uhp A基因与鱼类中的E. piscicida的致病性有关。