Abstract Rosa pimpinellifolia L. fruits (RPF) have great industrial potential due to the high presence of anthocyanins. In this work, microwave-assisted aqueous two-phase extraction (MA-ATPE) was applied to simultaneous extraction and purification of anthocyanins from RPF with ethanol/ammonium sulfate aqueous two-phase system (ATPS). MA-ATPE process was optimized by single-factor experiments and response surface methodology. The optimal anthocyanin yields were achieved in conditions of 0% HCl (w:w), 26.85% ethanol (w:w), 19.15% ammonium sulfate (w:w), liquid to solid ratio 40, 17.29 min, 60 °C and 400 W. Predicted anthocyanin yield of MA-ATPE at optimum conditions were determined as 1373.04 mg cyanidin 3-glucoside equivalent/g dry RPF. The purity of anthocyanin rich extract obtained by MA-ATPE was 1.65-fold greater than microwave assisted extraction using 80% ethanol (MAE). The compositional characterization of anthocyanins were carried out with HPLC-QTOF-MS. MA-ATPE had no obvious changes on anthocyanin composition when compared to MAE. Results indicated that the MA-ATPE could be a promising method for the extraction and preliminary purification of anthocyanins from different plant materials.

中文翻译：

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微波辅助水相两相萃取法同时分离和初步纯化小叶蔷薇果实中的花青素

摘要 Rosa pimpinellifolia L. Fruits (RPF) 由于花青素含量高而具有巨大的工业潜力。在这项工作中，微波辅助水相两相萃取 (MA-ATPE) 被应用到乙醇/硫酸铵水相两相系统 (ATPS) 中同时提取和纯化 RPF 中的花青素。MA-ATPE 工艺通过单因素实验和响应面方法进行了优化。在 0% HCl (w:w)、26.85% 乙醇 (w:w)、19.15% 硫酸铵 (w:w)、液固比 40、17.29 min、60 °C 和400 W。在最佳条件下，MA-ATPE 的预测花青素产量确定为 1373.04 mg 花青素 3-葡萄糖苷当量/g 干 RPF。MA-ATPE得到的富含花青素的提取物纯度为1。比使用 80% 乙醇 (MAE) 的微波辅助提取高 65 倍。花青素的成分表征采用 HPLC-QTOF-MS。与 MAE 相比，MA-ATPE 的花色苷成分没有明显变化。结果表明，MA-ATPE 可能是一种从不同植物材料中提取和初步纯化花青素的有前途的方法。