Developing effect methods for sensing N₂H₄ in the environmental and biological systems is particularly important as its terrible toxicity. However, most of the reported N₂H₄ fluorescent probes have aggregation-caused quenching (ACQ) effect, limited Stokes shift and short emission wavelengths, and thus these shortcomings restrain their comprehensive applications. In this work, we designed a first AIE N₂H₄ fluorescent probe Co-NH based on the α-cyanostilbene derivative. This probe Co-NH integrated intramolecular charge transfer (ICT) and AIE mechanisms. The probe Co-NH has remarkable aggregations with increasing proportion of water, and these aggregates show obviously redshift in emission with large Stokes shift (170 nm and 205 nm in 1,4-dixoane and pure water respectively). Importantly, Co-NH can sense N₂H₄ in 1,4-dioxane/H₂O (v/v = 9/1) solution with low LOD (0.101 μM), high selectivity and photostability. With the aids of fluorescence in solid-states, Co-NH has achieved in detecting N₂H₄ in TLC plates. In addition, Co-NH exhibits the ability for tracing N₂H₄ in living cells and zebrafish. We hope Co-NH is a useful tool for detection of N₂H₄ in various environments.

由于其可怕的毒性，开发在环境和生物系统中感测N ₂ H _4的效应方法尤其重要。然而，大多数报道的N ₂ H ₄荧光探针具有聚集引起的猝灭（ACQ）效应，有限的斯托克斯位移和短的发射波长，因此这些缺点限制了它们的全面应用。在这项工作中，我们设计了第一个基于α-氰基二苯乙烯衍生物的AIE N ₂ H ₄荧光探针Co - NH。该探针结合了Co - NH分子内的电荷转移（ICT）和AIE机制。探针公司- NH具有增加水的比例显着聚集，并且这些聚集体明显红移与斯托克斯频移大发射（170 nm和1,4- dixoane 205 nm和纯水分别）示出。重要的是，Co - NH可以在低LOD（0.101μM），高选择性和光稳定性的情况下检测1,4-二恶烷/ H ₂ O（v / v = 9/1）溶液中的N ₂ H ₄。借助固态荧光，Co - NH可检测TLC板中的N ₂ H ₄。此外，Co - NH具有追踪N ₂ H的能力₄在活细胞和斑马鱼中。我们希望Co - NH是在各种环境中检测N ₂ H ₄的有用工具。