Tea plant (Camellia sinensis (L.) O. Kuntze) is known to accumulate high concentrations of fluoride (F) in its leaves; however, the underlying mechanism of F accumulation remains unclear. The main objective of this study was to investigate the homeostatic self-defense mechanisms of tea leaves to F supplementation (0, 5, 20, and 50 mgL⁻¹) by metabolomics and ionomics. We identified a total of 96 up-regulated and 40 down-regulated metabolites in tea leaves treated with F. Of these different compounds, minor polypeptides, carbohydrates and amino acids played valuable roles in the F-tolerating mechanism of tea plant. After F treatments, the concentrations of sodium (Na), ferrum (Fe), manganese (Mn), and molybdenum (Mo) were significantly increased in tea leaves, whereas the aluminum (Al) was decreased. These findings suggest that the ionic balance and metabolites are attributable to the development of F tolerance, providing new insight into tea plant adaptation to F stress.

已知茶树（山茶（L.）O。Kuntze）在其叶片中会积聚高浓度的氟化物（F）。但是，F积累的潜在机制仍不清楚。这项研究的主要目的是研究茶叶补充F（0、5、20和50 mgL ^-1时的稳态自我防御机制）。），通过代谢组学和ionomics。我们在用F处理的茶叶中鉴定出总共96种上调的代谢产物和40种下调的代谢产物。在这些不同的化合物中，次要多肽，碳水化合物和氨基酸在茶树的F耐受机制中发挥了重要作用。经过F处理后，茶叶中钠（Na），铁（Fe），锰（Mn）和钼（Mo）的浓度显着增加，而铝（Al）则降低。这些发现表明，离子平衡和代谢产物可归因于F耐受性的发展，为茶树适应F胁迫提供了新的见识。