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Succession of Bacterial Communities in the Decomposition of Oats Straw in Two Soils with Contrasting Properties
Eurasian Soil Science ( IF 1.4 ) Pub Date : 2020-11-27 , DOI: 10.1134/s1064229320090112
O. V. Orlova , A. A. Kichko , E. V. Pershina , A. G. Pinaev , E. E. Andronov

Abstract

Significance of soil genesis for the composition and succession of bacterial communities during straw decomposition has been studied. Sterile grinded oat straw in nylon bags was placed in humus horizons of two soils (soddy-podzolic and chernozem; Albic Retisol (Abruptic, Ochric) and Haplic Chernozem (Pachic)) and incubated in the laboratory under constant hydrothermal conditions. On the basis of respiration dynamics (measured by absorption method) the stages of straw decomposition (early, middle and late) were specified. For each stage, the composition of bacterial communities was determined by high-performance sequencing of amplicon libraries of the variable region V4 of the 16S rRNA gene. It has been shown that carbon losses during decomposition of straw from the soddy-podzolic soil were higher than those from chernozem: 3913 ± 668 and 2981 ± 297 CO2 mg/m2 day, respectively. Phylogenetic analysis showed an increase in the diversity of the bacterial community in the process of straw decomposition: from 51 and 79 on the third day, up to 213 and 310 phylotypes from 17 phyla on the last day, for chernozem and soddy-podzolic soil, respectively. On the third day, Proteobacteria, Firmicutes, and Bacteroidetes were mainly present; on the 161st day, Actinobacteria were added. Soil had a significant impact on the taxonomic composition of straw communities. For the microbial community of straw formed on the basis of the microbiocenosis of soddy-podzolic soil the proportion of Proteobacteria was higher, but Bacteroidetes was lower, and the contribution of unidentified prokaryotes was more significant. Bacterial succession during straw decomposition had common features for both soils. The decomposition started with the quick consuming of easily available organic compounds of straw by copiotrophs, representatives of the Pseudomonas genus, the Stenotrophomonas genus from the family of Xanthomonaceae, and the Chitinophaga (Bacteroidia) genus. In addition to actinobacteria, the oligotrophs were represented by Firmicutes and part of Bacteroidetes. Throughout the entire observation period, probably due to the formation of available organic compounds during the decomposition of straw, the contribution of bacteria of the Rhizobiaceae family was significant.



中文翻译:

两种性质相反的燕麦秸秆分解过程中细菌群落的演替

摘要

研究了土壤发生对秸秆分解过程中细菌群落组成和演替的意义。将无菌研磨燕麦袋装在尼龙袋中,放在两种土壤(腐殖土和黑钙石; Albic Retisol(Abruptic,Ochric)和Haplic Chernozem(Pachic))的腐殖质层中,并在恒定的水热条件下在实验室中孵育。根据呼吸动力学(通过吸收法测量),确定了秸秆分解的阶段(早,中,晚)。对于每个阶段,通过对16S rRNA基因可变区V4的扩增子文库进行高效测序来确定细菌群落的组成。研究表明,在水土质土壤中秸秆分解过程中的碳损失高于黑钙土的碳损失:2毫克/平方米2分别。系统发育分析表明,在稻草分解过程中,细菌群落的多样性有所增加:从第三天的51和79,到最后一天的17种门的213和310种系统型,对于黑钙土和泥泞的土壤,分别。第三天,主要存在变形杆菌,硬毛菌和拟杆菌。在第161天,加入了放线菌。土壤对稻草群落的生物分类构成有重大影响。对于以泥泞土壤为基础的微生物群落形成的秸秆微生物群落而言,Proteobacteria的比例较高,而Bacteroidetes的比例较低,未鉴定的原核生物的贡献较大。两种土壤在秸秆分解过程中的细菌演替都有共同特征。假单胞菌属,黄单胞菌科的嗜单胞菌属和ChitinophagaBacteroidia)属。除放线菌外,寡养菌还以Firmicutes和部分拟杆菌属为代表。在整个观察期内,可能是由于秸秆分解过程中形成了有效的有机化合物,根瘤菌科细菌的贡献很大。

更新日期:2020-11-27
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