A fluorimetric analytical method using test strips has been fabricated for detecting Hg²⁺ ions in blood by using copper nanoclusters (Cu NCs) prepared via a biomineralization route. Unexpectedly, the as-prepared Cu NCs displayed greatly amplified red fluorescence once dispersed in DMSO, the intensity of which decreased specifically in the presence of Hg²⁺. Moreover, the resultant Cu NCs were deposited onto test strips to be further fast dried on superhydrophobic substrates in vacuum. The test strip-based fluorimetry can allow for the direct analysis of Hg²⁺ in blood in the linear concentration range of 0.10–1000 nM. Importantly, this solvent-enhanced fluorescence protocol for different metal probes such as Cu NCs promises extensive analysis applications for designing numerous fluorimetric platforms such as test strips.

中文翻译：

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荧光测试条，用于使用具有DMSO的荧光和稳定性的铜纳米簇对肉眼中的汞进行视觉评估

通过使用通过生物矿化途径制备的铜纳米团簇（Cu NCs），已经制造了使用测试条的荧光分析方法，用于检测血液中的Hg ²⁺离子。出乎意料的是，如此制备的Cu NCs分散在DMSO中后，显示出大大放大的红色荧光，其强度在存在Hg ²⁺的情况下特别降低。而且，将所得的Cu NCs沉积在测试条上，以在真空中在超疏水性基底上进一步快速干燥。基于测试条的荧光法可以直接分析Hg ²⁺在血液中的线性浓度范围为0.10–1000 nM。重要的是，这种用于溶剂的增强型荧光协议可用于不同的金属探针（例如Cu NCs），有望在设计多种荧光平台（例如测试条）时得到广泛的分析应用。