Mannitol has recently been reported to be effective in enhancing the antinociceptive efficacy of lidocaine. No single study to date, however, has compared diphenhydramine with and without mannitol for nociceptive processing as an alternative local anesthetic. In this study, we examined the antinociceptive efficacy enhancements of diphenhydramine when combined with mannitol. Male Sprague-Dawley rats weighing 230–260 g were used in a hot plate test to evaluate the antinociceptive effects of diphenhydramine. All chemicals were dissolved in isotonic normal saline and administered subcutaneously into the plantar surface of the right hind paw at 10 min before the hot plate test. A subcutaneous injection of 0.5% or 1% diphenhydramine produced significant inhibition of the withdrawal latency time compared with the vehicle treatment. Antinociceptive effects appeared 10 min after the diphenhydramine injections and persisted for over 30 min. The antinociceptive effects of 1% diphenhydramine were not statistically different from those of 1% lidocaine. Although a subcutaneous injection of a 0.5 M mannitol solution alone did not affect the withdrawal latency time, 1% diphenhydramine with 0.5 M mannitol significantly enhanced antinociception. A subcutaneous injection of 1% diphenhydramine with epinephrine (1 : 100,000) solution did not increase the antinociceptive effect of the diphenhydramine. These results suggest that diphenhydramine with mannitol can be used as an alternative local anesthetic.

中文翻译：

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甘露醇增强苯海拉明作为替代性局部麻醉药的镇痛作用

最近有报道称甘露醇可有效提高利多卡因的抗伤害感受功效。然而，迄今为止，没有一项单独的研究将苯海拉明与甘露醇与不加甘露醇进行比较，以作为替代性局部麻醉剂进行伤害性处理。在这项研究中，我们研究了与甘露醇联合使用时苯海拉明的抗伤害作用增强。重230-260 g的雄性Sprague-Dawley大鼠在热板试验中用于评估苯海拉明的镇痛作用。在热板试验前10分钟，将所有化学药品溶于等渗的生理盐水中，并皮下注射到右后爪的足底表面。与媒介物治疗相比，皮下注射0.5％或1％苯海拉明可显着抑制停药潜伏时间。苯海拉明注射后10分钟出现了镇痛作用，并持续了30分钟以上。1％苯海拉明的镇痛作用与1％利多卡因的镇痛作用无统计学差异。尽管仅皮下注射0.5 M甘露醇溶液不会影响撤药潜伏时间，但是1％苯海拉明与0.5 M甘露醇的作用明显增强了抗伤害感受性。皮下注射1％苯海拉明与肾上腺素（1：100,000）溶液不会增加苯海拉明的镇痛作用。这些结果表明苯海拉明与甘露醇可以用作替代的局部麻醉剂。1％苯海拉明的镇痛作用与1％利多卡因的镇痛作用无统计学差异。尽管仅皮下注射0.5 M甘露醇溶液不会影响撤药潜伏时间，但是1％苯海拉明与0.5 M甘露醇可显着增强止痛作用。皮下注射1％苯海拉明与肾上腺素（1：100,000）溶液不会增加苯海拉明的镇痛作用。这些结果表明苯海拉明与甘露醇可以用作替代的局部麻醉剂。1％苯海拉明的镇痛作用与1％利多卡因的镇痛作用无统计学差异。尽管仅皮下注射0.5 M甘露醇溶液不会影响撤药潜伏时间，但是1％苯海拉明与0.5 M甘露醇可显着增强止痛作用。皮下注射1％苯海拉明与肾上腺素（1：100,000）溶液不会增加苯海拉明的镇痛作用。这些结果表明苯海拉明与甘露醇可以用作替代的局部麻醉剂。000）溶液并没有增加苯海拉明的镇痛作用。这些结果表明苯海拉明与甘露醇可以用作替代的局部麻醉剂。000）溶液并没有增加苯海拉明的镇痛作用。这些结果表明苯海拉明与甘露醇可以用作替代的局部麻醉剂。