The broad application of large-scale single-cell RNA profiling in plants has been restricted by the prerequisite of protoplasting. We recently found that the Arabidopsis nucleus contains abundant polyadenylated mRNAs, many of which are incompletely spliced. To capture the isoform information, we combined 10x Genomics and Nanopore long-read sequencing to develop a protoplasting-free full-length single-nucleus RNA profiling method in plants. Our results demonstrated using Arabidopsis root that nuclear mRNAs faithfully retain cell identity information, and single-molecule full-length RNA sequencing could further improve cell type identification by revealing splicing status and alternative polyadenylation at single-cell level.

中文翻译：

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植物中的单核全长RNA谱分析结合了同工型信息以促进细胞类型鉴定

大规模单细胞RNA分析在植物中的广泛应用已受到原生质体形成的先决条件的限制。我们最近发现，拟南芥核含有丰富的聚腺苷酸mRNA，其中许多是不完全剪接的。为了捕获同工型信息，我们结合了10x基因组学和Nanopore长读测序技术，开发了一种无需原生质体移植的全长单核RNA分析方法。我们的研究结果表明，使用拟南芥根能够忠实地保留细胞身份信息，而单分子全长RNA测序可通过揭示单细胞水平的剪接状态和其他聚腺苷酸化作用来进一步改善细胞类型识别。