The detection and diagnosis of the opportunistic fungi Scedosporium spp. and Lomentospora prolificans still relies mainly on low-sensitive culture-based methods. This fact is especially worrying in Cystic Fibrosis (CF) patients in whom these fungal species are frequently isolated and may increase the risk of suffering from an infection or other health problems. Therefore, with the purpose of developing a serologic detection method for Scedosporium/Lomentospora, four different Scedosporium boydii protein extracts (whole cell protein extract, secretome, total cell surface and conidial surface associated proteins) were studied by ELISA to select the most useful for IgG detection in sera from CF patients. The four extracts were able to discriminate the Scedosporium/Lomentospora-infected from Aspergillus-infected and non-infected patients. However, the whole cell protein extract was the one selected, as it was the one with the highest output in terms of protein concentration per ml of fungal culture used, and its discriminatory capacity was the best. The ELISA test developed was then assayed with 212 sera from CF patients and it showed to be able to detect Scedosporium spp. and Lomentospora prolificans with very high sensitivity and specificity, 86%–100% and 93%–99%, respectively, depending on the cut-off value chosen (four values were proposed A_450nm= 0.5837, A_450nm= 0.6042, A_450nm= 0.6404, and A_450nm= 0.7099). Thus, although more research is needed to reach a standardized method, this ELISA platform offers a rapid, low-cost and easy solution to detect these elusive fungi through minimally invasive sampling, allowing the monitoring of the humoral response to fungal presence.

机会性真菌的检测与诊断 鞘孢菌spp。和产多孢子虫仍然主要依靠低敏感性的基于文化的方法。对于经常分离这些真菌种类的囊性纤维化（CF）患者，这一事实尤其令人担忧，并且可能增加感染或其他健康问题的风险。因此，以开发一种血清学检测方法为目的鞘孢菌/孢子虫，四个不同 伯氏假单胞菌通过ELISA研究了蛋白质提取物（全细胞蛋白质提取物，分泌蛋白组，总细胞表面和分生孢子表面相关的蛋白质），以选择对CF患者血清中最有用的IgG检测。四种提取物能够区分鞘孢菌/孢子虫-感染自 曲霉菌感染和未感染的患者。但是，全细胞蛋白质提取物是一种选择，因为它是每毫升所用真菌培养物中蛋白质浓度最高的一种，并且其鉴别能力最好。然后用来自CF患者的212份血清进行了ELISA测试，结果表明该试剂盒能够检测到鞘孢菌spp。和产多孢子虫具有非常高的灵敏度和特异性，分别取决于选择的截止值（分别为86％–100％和93％–99％）（建议四个值分别为A _450nm = 0.5837，A _450nm = 0.6042，A _450nm = 0.6404和甲_在450nm = 0.7099）。因此，尽管需要更多的研究来达到标准化的方法，但该ELISA平台提供了一种快速，低成本和简便的解决方案，可通过微创采样来检测这些难闻的真菌，从而监控对真菌存在的体液反应。