Increasing knowledge of the role of the intestinal microbiome in human health and well-being has resulted in increased interest in prebiotics, mainly oligosaccharides of various origins. To date, there are no reports in the literature on the prebiotic properties of oligosaccharides produced by the hydrolysis of pure fungal α-(1→3)-glucan. The aim of this study was to prepare α-(1→3)-glucooligosaccharides (α-(1→3)-GOS) and to perform initial evaluation of their prebiotic potential. The oligosaccharides were obtained by acid hydrolysis of α-(1→3)-glucan isolated from the fruiting bodies of Laetiporus sulphureus and then, characterized by HPLC. Fermentation of α-(1→3)-GOS and reference prebiotics was compared in in vitro pure cultures of Lactobacillus, Bifidobacterium, and enteric bacterial strains. A mixture of α-(1→3)-GOS, notably with a degree of polymerization of 2 to 9, was obtained. The hydrolysate was utilized for growth by most of the Lactobacillus strains tested and showed a strong bifidogenic effect, but did not promote the growth of Escherichia coli and Enterococcus faecalis. α-(1→3)-GOS proved to be effective in the selective stimulation of beneficial bacteria and can be further tested to determine their prebiotic functionality.

中文翻译：

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通过酸水解硫磺粉菌中的 α-(1→3)-葡聚糖获得的低聚糖的益生元潜力：一项初步研究

随着人们对肠道微生物群在人类健康和福祉中作用的认识不断增加，人们对益生元（主要是各种来源的寡糖）的兴趣增加。迄今为止，文献中还没有关于由纯真菌α-(1→3)-葡聚糖水解产生的低聚糖的益生元特性的报道。本研究的目的是制备 α-(1→3)-葡萄糖寡糖 (α-(1→3)-GOS) 并对其益生元潜力进行初步评估。寡糖是通过酸解从硫磺粉菌子实体中分离得到的α-(1→3)-葡聚糖，然后用高效液相色谱法表征。在乳酸杆菌、双歧杆菌和肠道细菌菌株的体外纯培养物中比较了 α-(1→3)-GOS 和参考益生元的发酵。α-(1→3)-GOS的混合物，尤其是获得了聚合度为 2 至 9 的聚合物。水解产物被大多数被测乳酸杆菌菌株用于生长，并显示出强烈的双歧杆菌作用，但不促进大肠杆菌和粪肠球菌的生长。α-(1→3)-GOS 被证明在选择性刺激有益细菌方面是有效的，可以进一步测试以确定它们的益生元功能。