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Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912
Scientific Reports ( IF 4.6 ) Pub Date : 2020-11-26 , DOI: 10.1038/s41598-020-77402-6
Carole A Llewellyn 1 , Carolyn Greig 1 , Alla Silkina 1 , Bethan Kultschar 1 , Matthew D Hitchings 2 , Garry Farnham 3
Affiliation  

The “UV sunscreen” compounds, the mycosporine-like amino acids (MAAs) are widely reported in cyanobacteria and are known to be induced under ultra-violet (UV) light. However, the impact of far red (FR) light on MAA biosynthesis has not been studied. We report results from two experiments measuring transcriptional regulation of MAA and aromatic amino acid pathways in the filamentous cyanobacterium Chlorogloeopsis fritschii PCC 6912. The first experiment, comparing UV with white light, shows the expected upregulation of the characteristic MAA mys gene cluster. The second experiment, comparing FR with white light, shows that three genes of the four mys gene cluster encoding up to mycosporine-glycine are also upregulated under FR light. This is a new discovery. We observed corresponding increases in MAAs under FR light using HPLC analysis. The tryptophan pathway was upregulated under UV, with no change under FR. The tyrosine and phenylalanine pathways were unaltered under both conditions. However, nitrate ABC transporter genes were upregulated under UV and FR light indicating increased nitrogen requirement under both light conditions. The discovery that MAAs are upregulated under FR light supports MAAs playing a role in photon dissipation and thermoregulation with a possible role in contributing to Earth surface temperature regulation.



中文翻译:

蓝细菌 Chlorogloeopsis fritschii PCC 6912 中类菌孢素氨基酸和芳香族氨基酸转录组对紫外线和远红光的响应

“紫外线防晒剂”化合物,类菌孢素氨基酸 (MAA) 在蓝藻中被广泛报道,并且已知在紫外线 (UV) 光下会被诱导。然而,尚未研究远红 (FR) 光对 MAA 生物合成的影响。我们报告了测量丝状蓝藻Chlorogloeopsis fritschii PCC 6912中 MAA 和芳香族氨基酸途径的转录调控的两个实验的结果。第一个实验将紫外线与白光进行比较,显示了特征性 MAA my s 基因簇的预期上调。第二个实验,比较 FR 与白光,结果表明四个mys 的三个基因在 FR 光下,编码至 mycosporine-glycine 的基因簇也被上调。这是一个新的发现。我们使用 HPLC 分析观察到在 FR 光下 MAA 的相应增加。色氨酸途径在 UV 下上调,在 FR 下没有变化。酪氨酸和苯丙氨酸途径在两种条件下均未改变。然而,硝酸盐 ABC 转运蛋白基因在 UV 和 FR 光下上调,表明在两种光照条件下氮需求增加。MAA 在 FR 光下上调的发现支持 MAA 在光子耗散和温度调节中发挥作用,并可能有助于地球表面温度调节。

更新日期:2020-11-27
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