ABSTRACT: The cyclopoid Apocyclops royi (Lindberg 1940) and the calanoid Pseudodiaptomus annandalei (Sewell 1919) are 2 tropical copepods suspected of having the capability to biosynthesize the physiologically important n-3 polyunsaturated fatty acids (n-3 PUFAs) eicosapentaenoic acid (C20:5n-3, EPA) and docosahexaenoic acid (C22:6n-3, DHA). We demonstrated this suspected ability using ¹³C₁₈ α-linolenic acid (C18:3n-3, ALA) fed to the copepods through liposomes and a subsequent fatty acid (FA) analysis by GC-MS at 3 different time points (0, 24, and 48 h). Two different diets were applied post liposome exposure: baker’s yeast Saccharomyces cerevisiae and the microalga Dunaliella tertiolecta. For both copepods, further elongated and desaturated ¹³C n-3 PUFAs were found at all time points. At T_48h, A. royi and P. annandalei contained ¹³C-labelled DHA contents (±SD) of 1.3 ± 0.2 and 0.7 ± 0.3 µg ¹³C-FA mgC_copepod^-1 when fed baker’s yeast, respectively, and 1.2 ± 0.1 and 1.6 ± 0.5 µg ¹³C-FA mgC_copepod^-1 when fed D. tertiolecta, respectively, with significant differences observed only between P. annandalei diet treatments. The ¹³C-labelled EPA content of A. royi and P. annandalei at T_48h was 0.6 ± 0.4 and 0.7 ± 0.4 µg ¹³C-FA mgC_copepod^-1 when fed baker’s yeast and 0.8 ± 0.2 and 0.3 ± 0.1 µg ¹³C-FA mgC_copepod^-1 when fed D. tertiolecta, with significant differences only between copepods fed D. tertiolecta. A. royi and P. annandalei exhibited an ability to produce n-3 PUFAs from the precursor ALA in comparatively large quantities. This ability enables these 2 species to inhabit habitats characterized by PUFA-poor particulate material.

中文翻译：

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热带co足类Apocyclops royi和Pseudodiaptomus annandalei对PUFA苦咸水生境的生化适应

摘要：摆线体Apocyclops royi（Lindberg 1940）和Calanoid Pseudodiaptomus annandalei（Sewell 1919）是2种热带co足类动物，被怀疑具有生物合成生理上重要的n-3多不饱和脂肪酸（n-3 PUFAs）二十碳五烯酸（C20： 5n-3，EPA）和二十二碳六烯酸（C22：6n-3，DHA）。我们证明了这种怀疑使用能力¹³ Ç ₁₈ α亚麻酸（C18：3n一3，ALA）馈送到桡足类通过脂质体和通过GC-MS的后续脂肪酸（FA）的分析在3个不同的时间点（0，24和48小时）。脂质体暴露后使用两种不同的饮食：面包酵母啤酒酵母和微藻杜氏藻。对于这两种co足类动物，在所有时间点都发现了进一步伸长和去饱和的¹³ C n-3 PUFA。在T _48小时，A. royi和P.白虾含有¹³ 1.3±0.2和0.7±0.3微克C标记的DHA含量（±SD）¹³ C-FA MGC_桡足类^-1进料时的面包酵母，分别和1.2±0.1和1.6±0.5μg的¹³ C-FA MGC_桡足类^-1进料时杜氏盐藻，分别与显著差异仅之间观察到P.白虾饮食疗法。的¹³的C标记的EPA含量A. royi和P.白虾在T _48小时为0.6±0.4和0.7±0.4微克¹³ C-FA MGC_桡足类^-1时馈面包酵母和0.8±0.2和0.3±0.1微克¹³ C-FA MGC_桡足类^-1进料时杜氏盐藻，仅在pe足。足纲between之间有显着差异。A. royi和P. annandalei具有从前体ALA大量生产n-3 PUFA的能力。这种能力使这2个物种能够居住在以PUFA贫乏的颗粒物质为特征的栖息地中。