ABSTRACT: Zooplankton production estimates are necessary to understand the availability and transfer of energy to higher trophic levels in marine food webs. Methods have been developed to quantify zooplankton production; however, they are difficult to compare as they focus on single species, groups, stages, or size classes of zooplankton. We compared 2 methods for estimating crustacean production: the chitobiase method (based on a crustacean moulting enzyme), and 3 empirical growth rate models (Huntley-Lopez, Hirst-Lampitt, and Hirst-Bunker) applied to optically resolved mesozooplankton normalized biomass size spectra (NBSS). Mesozooplankton net samples were collected between March and August of 2010 and 2011 in Saanich Inlet (British Columbia, Canada) and analyzed in the laboratory using microscopy and a bench-top laser optical particle counter (lab-LOPC). Microscope and lab-LOPC estimates of abundance and biomass were in close agreement. Crustacean production estimates were highest using Huntley-Lopez (0.20-185.3 mg C m^-3 d^-1), followed by Hirst-Bunker (0 .01-18.3 mg C m^-3 d^-1), chitobiase (0.05-15.6 mg C m^-3 d^-1), and Hirst-Lampitt (0.03-14.3 mg C m^-3 d^-1). Hirst-Lampitt-, Hirst-Bunker-, and chitobiase-based estimates of crustacean production and trophic transfer efficiency (TTE) yielded similar patterns/magnitude, while the Huntley-Lopez model was more variable. Estimates showed stronger agreement in 2011 than in 2010, attributed to the shift from El Niño to La Niña conditions. We highlight similarities/differences associated with these techniques and suggest that Hirst-Bunker estimates of production and TTE are most consistent with chitobiase-based values.

中文翻译：

---

Saanich Inlet（加拿大不列颠哥伦比亚省）使用壳聚糖酶和生物量大小光谱方法对中游浮游动物产量估算值的比较

摘要：浮游动物的产量估算对于了解海洋食物网中能量的​​可用性和较高营养级的传递是必要的。已经开发出量化浮游动物产量的方法；但是，由于它们只关注浮游动物的单个物种，群体，阶段或大小类别，因此很难进行比较。我们比较了两种估算甲壳类动物产量的方法：壳聚糖酶法（基于甲壳类蜕皮酶）和3种经验增长率模型（Huntley-Lopez，Hirst-Lampitt和Hirst-Bunker），该方法用于光学解析的中膜浮游生物归一化生物量粒径谱（NBSS）。Mesozooplankton净样品是在2010年3月至2011年8月之间于Saanich Inlet（不列颠哥伦比亚省，加拿大），并在实验室中使用显微镜和台式激光光学粒子计数器（lab-LOPC）进行了分析。显微镜和实验室-LOPC估计的丰度和生物量非常吻合。使用Huntley-Lopez（0.20-185.3 mg C m^-3 d ^-1），然后是Hirst-Bunker（0 .01-18.3 mg C m ^-3 d ^-1），壳聚糖酶（0.05-15.6 mg C m ^-3 d ^-1）和Hirst-Lampitt（0.03- 14.3mg C m ^-3 d ^-1）。基于Hirst-Lampitt，Hirst-Bunker和壳聚糖酶的甲壳类动物产量和营养传递效率（TTE）的估计得出相似的模式/幅度，而Huntley-Lopez模型的变化更大。据估计，由于从厄尔尼诺现象向拉尼娜现象的转变，2011年比2010年更加一致。我们强调了与这些技术相关的相似性/差异，并建议Hirst-Bunker对生产量和TTE的估算与基于壳聚糖酶的值最一致。