BACKGROUND & AIMS Intrahepatic cholangiocarcinoma (ICC) has over the last 10 years become the focus of increasing concern largely due to its rising incidence and high mortality rates worldwide. Microcystin-leucine-arginine (MC-LR) has been reported to be carcinogenic, but there are no data on the linkage between MC-LR and ICC. This study aimed to explore whether the content levels of MC-LR in the tumour tissues of ICC patients be associated with the prognosis and if so, to characterize the mechanism in ICC cells. METHODS We conducted a retrospective study to evaluate the prognostic value of MC-LR in ICC after resection. All patients were divided into two groups according to the content of MC-LR in tumour via immunohistochemistry: low-MC-LR group (n = 28) and high-MC-LR group (n = 30). RESULTS Multivariate analysis showed high-MC-LR level was the prognostic factor for OS and RFS after hepatectomy (P = .011 and .044). We demonstrated that MC-LR could promote the survival of human ICC cell lines and SET was identified as an important mRNA in the progression via RNA array. CONCLUSIONS We provide evidence that MC-LR was an independent prognostic factor for ICC in humans by modulating the expression of SET in human ICC cells.

中文翻译：

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较高含量的微囊藻毒素-亮氨酸-精氨酸通过调节SET促进肝内胆管癌细胞存活，导致患者预后较差

背景和目的 肝内胆管癌 (ICC) 在过去 10 年中已成为越来越受关注的焦点，这主要是由于其在世界范围内的发病率上升和高死亡率。据报道，微囊藻毒素-亮氨酸-精氨酸 (MC-LR) 具有致癌性，但没有关于 MC-LR 与 ICC 之间联系的数据。本研究旨在探讨ICC患者肿瘤组织中MC-LR的含量水平是否与预后相关，如果是，则表征ICC细胞中的机制。方法 我们进行了一项回顾性研究，以评估 MC-LR 在 ICC 切除后的预后价值。所有患者根据肿瘤中MC-LR的含量通过免疫组织化学分为两组：低MC-LR组（n = 28）和高MC-LR组（n = 30）。结果 多变量分析显示，高 MC-LR 水平是肝切除术后 OS 和 RFS 的预后因素（P = .011 和 .044）。我们证明 MC-LR 可以促进人 ICC 细胞系的存活，并且通过 RNA 阵列将 SET 鉴定为进展中的重要 mRNA。结论 我们提供证据表明 MC-LR 是人类 ICC 的独立预后因素，通过调节人类 ICC 细胞中 SET 的表达。