Male accessory gland (MAG) secretory proteins affect female reproductive physiology and behaviour when they are delivered to the female at the time of mating along with sperm. In our study, proteomic approaches were used to identify MAG proteins of Leucinodes orbonalis, a monophagous and destructive pest of brinjal. A set of 117 and 186 MAG spots were observed in virgin and mated males, respectively, using two‐dimensional gel electrophoresis (2D‐GE). Among the identified spots, 49 are unique to the virgin, whereas 118 are unique to mated MAGs, with 68 spots found to be common. The differentially expressed MAG proteins, 14 are up‐regulated, and 16 are down‐regulated in virgin after mating. We used matrix‐assisted laser desorption ionization (MALDI)–mass spectrometry (MS) to identify the 13 unique proteins in the MAG of virgin L. orbonalis and analysed the data of the Swiss‐Prot database using the Mascot search engine. The proteins were identified as proteolysis regulators, lipid transporters, olfactory proteins, metabolism‐related proteins, DNA‐binding proteins, and hexamerins. This is the first report on the gel‐based protein analysis of L. orbonalis MAGs.

中文翻译：

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比较原始的和交配的亮白蛛男性的副腺的蛋白质谱

雄性附属腺（MAG）分泌蛋白在与精子交配时传递给雌性时，会影响雌性生殖生理和行为。在我们的研究中，蛋白质组学方法被用于鉴定白芥的MAG蛋白，茄子的单相和破坏性害虫。使用二维凝胶电泳（2D-GE），分别在处女和交配的雄性中观察到一组117和186个MAG点。在识别出的斑点中，49个是处女所独有的，而118个是交配MAGs所独有的，其中68个是常见的。差异表达的MAG蛋白在交配后的原始蛋白中有14个上调，有16个下调。我们使用基质辅助激光解吸电离（MALDI）-质谱法（MS）来鉴定纯天然L. orbonalis MAG中的13种独特蛋白质并使用Mascot搜索引擎分析了Swiss-Prot数据库的数据。这些蛋白被鉴定为蛋白水解调节剂，脂质转运蛋白，嗅觉蛋白，代谢相关蛋白，DNA结合蛋白和六聚体蛋白。这是有关桔梗MAGs的基于凝胶的蛋白质分析的第一份报告。