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Evaluation of natural dyes Curcuma longa and Nyctanthes arbor‐tristis with different mordants on plant tissues under fluorescence microscopy
Microscopy Research and Technique ( IF 2.5 ) Pub Date : 2020-11-26 , DOI: 10.1002/jemt.23650
Nikita G Shet Verenkar 1 , Krishnan Sellappan 1
Affiliation  

The study reveals the effect of natural dyes Curcuma longa (rhizome) and Nyctanthes arbor‐tristis (corolla tube) on various plant tissues under fluorescence microscopy. The results indicated that the auto‐fluorescence was seen in some of the tissues of unstained monocot and dicot stem sections. The use of Curcuma longa dye along with different chemical mordants leads to various colors and intensity of fluorescence under different excitation filters (violet: 400–450; blue: 450–500; green: 500–570; yellow: 570–610 and red: 610–750 nm) examined. The extract of Nyctanthes arbor‐tristis along with the mordants lead to bluish‐orange fluorescence of vascular tissues under violet excitation filter. Among the dyes evaluated, Nyctanthes arbor‐tristis showed better fluorescence than the Curcuma longa dye. Hence, the dye extracted from the corolla tube of Nyctanthes arbor‐tristis is a potential natural dye and could be used as biological stain for staining plant tissues.

中文翻译:

荧光显微镜下对植物组织中不同媒染剂的天然染料姜黄和 Nyctanthes arbor-tristis 的评价

该研究揭示了天然染料Curcuma longa(根茎)和Nyctanthes arbor-tristis(花冠管)在荧光显微镜下对各种植物组织的影响。结果表明,在未染色的单子叶植物和双子叶植物茎切片的一些组织中观察到自发荧光。使用姜黄450-500;绿色:500-570;黄色:;蓝色400-450:具有不同化学媒染剂导致各种颜色和荧光的强度下不同的激励滤光器(紫色染料沿着和570-610红: 610–750 nm) 检查。Nyctanthes arbor-tristis的提取物连同媒染剂在紫色激发滤光片下导致血管组织的蓝橙色荧光。在评价的染料中,Nyctanthes arbor-tristis显示出比Curcuma longa染料更好的荧光。因此,从Nyctanthes arbor-tristis花冠管中提取的染料是一种潜在的天然染料,可用作植物组织染色的生物染料。
更新日期:2020-11-26
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