Free bilirubin level is a significant indicator for the diagnosis of jaundice related diseases. Herein, ceria nanocubes are synthesized as catalyst for the direct electrochemical sensor of free bilirubin. The sensor is fabricated by simply modifying screen-printed electrodes with ceria nanocubes and carbon blacks. Owing to the catalytical activity of ceria nanocubes and conductivity of carbon blacks, this sensor can detect free bilirubin with high sensitivity and selectivity in a short response time. Under the optimized condition, the current of DPV peak is proportional to the bilirubin concentration up to 100 μM, with a LOD of 0.1 μM in phosphate buffer and 1 μM in human serum sample. The non-enzymatic sensor also shows good reproducibility and stability at room temperature. Moreover, only 5 μL test sample is required, the small sample consumption may facilitate the blood collection procedure. The sensor could be a potential candidate device for the point of care testing on free bilirubin in clinic.

游离胆红素水平是诊断黄疸相关疾病的重要指标。在本文中，氧化铈纳米立方体被合成为催化剂用于游离胆红素的直接电化学传感器。该传感器是通过简单地用氧化铈纳米立方体和炭黑修改丝网印刷电极来制造的。由于氧化铈纳米立方体的催化活性和炭黑的电导率，该传感器可以在短响应时间内以高灵敏度和选择性检测游离胆红素。在最佳条件下，DPV峰的电流与胆红素浓度（最高100 µM）成正比，在磷酸盐缓冲液中的LOD为0.1 µM，在人血清样品中的LOD为1 µM。非酶传感器在室温下也显示出良好的重现性和稳定性。此外，只需要5 µL的测试样品，少量的样品消耗可以促进血液采集程序。该传感器可能是用于临床中游离胆红素的即时检测的潜在候选设备。