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Effect of zoledronic acid and graphene oxide on the physical and in vitro properties of injectable bone substitutes
Biomaterials Advances ( IF 7.9 ) Pub Date : 2020-11-27 , DOI: 10.1016/j.msec.2020.111758
Öznur Demir-Oğuz , Duygu Ege

The aim of this work was to develop injectable bone substitutes (IBS) consisting of zoledronic acid (ZOL) and graphene oxide (GO) for the treatment of osteoporosis and metastasis. The powder phase was consisting of tetra calcium phosphate (TTCP), dicalcium phosphate dihyrate (DCPD) and calcium sulfate dihyrate (CSD), while the liquid phase comprised of methylcellulose (MC), gelatin and sodium citrate dihyrate (SC), ZOL and GO. The structural analysis of IBS samples was performed by Fourier Transform Infrared Spectroscopy (FTIR). Injectability, setting time and mechanical strength were investigated. Additionally, in vitro properties of synthesized IBS were analyzed by means of bioactivity, ZOL release, degradation, pH variation, PO43− ion release and cell studies. Overall, all IBS exhibited excellent injectability results with no phase separation. The setting time of the IBS was prolonged with ZOL incorporation while the prolonging effect decreased with GO incorporation. The mechanical properties decreased with ZOL addition and increased with the incorporation of GO. The maximum compressive strength was found as 25.73 MPa for 1.5GO0ZOL incorporated IBS. In vitro results showed that ZOL and GO loaded IBS also revealed clinically suitable properties with controlled release of ZOL, pH value and PO43− ions. In in vitro cell studies, both the inhibitory effect of ZOL and GO loaded IBS on MCF-7 cells and proliferative effect on osteoblast cells were observed. Moreover, the prepared IBS led to proliferation, differentiation and mineralization of osteoblasts. The results are encouraging and support the conclusion that developed IBS have promising physical and in vitro properties which needs to be further validated by gene expression and in vivo studies.



中文翻译:

唑来膦酸和氧化石墨烯对可注射骨替代物的物理和体外特性的影响

这项工作的目的是开发由唑来膦酸(ZOL)和氧化石墨烯(GO)组成的可注射骨替代物(IBS),用于治疗骨质疏松和转移。粉末相由磷酸四钙(TTCP),磷酸二氢二钙(DCPD)和硫酸二氢钙(CSD)组成,而液相由甲基纤维素(MC),明胶和柠檬酸二氢钠(SC),ZOL和GO组成。IBS样品的结构分析通过傅里叶变换红外光谱(FTIR)进行。研究了可注射性,凝固时间和机械强度。此外,还通过生物活性,ZOL释放,降解,pH变异,PO 4 3-来分析合成IBS的体外特性。离子释放和细胞研究。总体而言,所有IBS均显示出优异的可注射性结果,且无相分离。ZOL掺入可延长IBS的凝固时间,而GO掺入可降低延长作用。力学性能随ZOL的添加而降低,随GO的引入而增加。发现掺入1.5GO0ZOL的IBS的最大抗压强度为25.73MPa。体外结果表明,ZOL和GO负载的IBS还具有可控制释放ZOL,pH值和PO 4 3-的临床特性。离子。在体外细胞研究中,观察到ZOL和GO负载的IBS对MCF-7细胞的抑制作用以及对成骨细胞的增殖作用。而且,制备的IBS导致成骨细胞的增殖,分化和矿化。该结果令人鼓舞,并支持以下结论:发达的IBS具有有希望的物理和体外特性,需要通过基因表达和体内研究进一步验证。

更新日期:2020-12-10
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