Rapid and effective repair of epithelial tissue is desirable for improving the success rate of operation and reducing postoperative complications. Hydrogel is a widely studied wound repair material, especially as a wound dressing for damaged epithelial tissue. Based on the catalytic effect of thrombin on fibrinogen, in this study, a three-dimensional fibrin gel which of adequate epithelial cell compatibility was constructed by using thrombin and fibrinogen under the cross-linking action of calcium ion. Immunofluorescence staining and hematoxylin-eosin (H&E) staining showed that bone marrow mesenchymal stem cell (BMSC) was embedded in fibrin gel. Furthermore, vascular endothelial growth factor (VEGF) was used to induce BMSC to differentiate into CD31+ and vWF+ endothelial cell (EC) in fibrin gel. The results showed that the fibrin gel surface may effectively promote the adhesion and proliferation of EC and smooth muscle cell (SMC). After 15 days of culture, it was found that the BMSC embedded in the hydrogel had differentiated into EC. The results of in vivo skin wound experiment in rats further proved that the fibrin gel containing BMSC could promote wound healing and repair, and showed the potential to promote neovascularization at the injured site. The construction method of hydrogel materials proposed in this study has potential application value in the field of regenerative medicine.

为了提高手术成功率并减少术后并发症，需要快速有效地修复上皮组织。水凝胶是被广泛研究的伤口修复材料，尤其是作为受损上皮组织的伤口敷料。基于凝血酶对纤维蛋白原的催化作用，本研究利用凝血酶和纤维蛋白原在钙离子的交联作用下构建了具有充分上皮细胞相容性的三维纤维蛋白凝胶。免疫荧光染色和苏木精-伊红（H＆E）染色显示，骨髓间充质干细胞（BMSC）嵌入在纤维蛋白凝胶中。此外，使用血管内皮生长因子（VEGF）诱导BMSC在纤维蛋白凝胶中分化为CD31 +和vWF +内皮细胞（EC）。结果表明，纤维蛋白凝胶表面可有效促进EC和平滑肌细胞（SMC）的粘附和增殖。培养15天后，发现包埋在水凝胶中的BMSC已经分化成EC。结果在大鼠体内皮肤伤口实验中进一步证明，含BMSC的纤维蛋白凝胶可促进伤口愈合和修复，并显示出促进受伤部位新血管形成的潜力。本研究提出的水凝胶材料的构建方法在再生医学领域具有潜在的应用价值。