In this work, a biocompatible monolithic column based micro-solid-phase extraction (µ-SPE) method was developed for biological fluid analysis. A novel nanoparticle-based polyacrylonitrile monolithic column (C30 NP-PMC) was fabricated by incorporating triacontyl (C30) modified silica nanoparticles (NPs) into the polyacrylonitrile monolithic matrix through thermally induced phase separation. With efficient mass transfer and sorption capacity, C30 NP-PMC exhibited outstanding performance for the extraction of carotenoids and fat-soluble vitamins (FSVs) from human serum samples, superior to commercial C18 cartridges as well as liquid-liquid extraction (LLE) method. Under optimal conditions, the proposed µ-SPE method coupled with high-performance liquid chromatography-diode array detection (HPLC-DAD) achieved satisfactory limits of detection (LODs) (1.5-75.0 ng/mL) and good recoveries (85.0-106.5 %) with relative standard deviations (RSDs) of less than 12.1% by consuming lower sorbent (35.0 mg) and organic solvent (0.8 mL). Successful application of the developed method demonstrated the great potential of such monolithic sorbents for efficient isolation and preconcentration of trace analytes from blood samples.

在这项工作中，开发了一种基于生物相容性整体柱的微固相萃取（μ-SPE）方法用于生物流体分析。通过热诱导相分离将三aco烯基（C30）改性的二氧化硅纳米颗粒（NPs）引入聚丙烯腈整体基质中，制得了一种新型的基于纳米颗粒的聚丙烯腈整体柱（C30 NP-PMC）。凭借有效的传质和吸附能力，C30 NP-PMC在从人血清样品中提取类胡萝卜素和脂溶性维生素（FSV）方面表现出卓越的性能，优于商用C18柱以及液-液提取（LLE）方法。在最佳条件下，所提出的μ-SPE方法与高效液相色谱-二极管阵列检测（HPLC-DAD）结合使用可达到令人满意的检测限（LOD）（1。（5-75.0 ng / mL）和良好的回收率（85.0-106.5％），通过使用较低的吸附剂（35.0 mg）和有机溶剂（0.8 mL）的相对标准偏差（RSD）小于12.1％。所开发方法的成功应用证明了这种整体式吸附剂在从血样中有效分离和预富集痕量分析物的巨大潜力。