The present study was designed to explore the in vivo-antioxidant capacity and the probable mechanism of AAPs-H, prepared from Auricularia auricula polysaccharides with the optimal extraction conditions by Box–Behnken design and acid hydrolysis, using Caenorhabditis elegans as a model organism. The effects of AAPs-H on the locomotion behavior, life span, antioxidant-related enzymes activities, and antioxidants levels in C. elegans were studied. Furthermore, the potentials of AAPs-H in up-regulating the expression of antioxidant-related genes in C. elegans, such as skn-1, sod-3 and sir-2.1, were also discussed. AAPs-H demonstrated a highly significant protective effect against the damage caused by paraquat, could significantly increase U-Turn frequency of worms (p < 0.01), extend their lifespan, enhance antioxidant systems including GR by 63.96% (p < 0.05), GSH-Px by 71.16% (p < 0.01), SOD by 78.65% (p < 0.01) and CAT by 98.52% (p < 0.01), increase the level of GSH by 28.12% (p < 0.05), and decrease the level of MDA by 39.29% (p < 0.01). The qRT-PCR results showed that AAPs-H could up regulate mRNA expression levels of skn-1, sod-1, sod-2, sod-3 and sir-2.1 in wild-type C. elegans (>1.6 fold) when treated with the concentration of 0.4 mg/mL (p < 0.05 or p < 0.01). Our studies provide evidence that AAPs-H improves antioxidant defense system, and up-regulation of oxidative stress related genes for prevention of stress damage in C. elegans.

本研究的目的是要探索的体内-antioxidant容量和的AAP-H的可能机制，从制备的黑木耳与由箱Behnken法设计和酸水解的最佳提取条件的多糖，用秀丽隐杆线虫作为模式生物。研究了AAPs-H对秀丽隐杆线虫运动行为，寿命，抗氧化剂相关酶活性和抗氧化剂水平的影响。此外，AAPs-H可能上调秀丽隐杆线虫中抗氧化剂相关基因的表达，如skn-1，sod-3和sir-2.1，也进行了讨论。AAPs-H表现出对百草枯造成的损害具有极显着的保护作用，可以显着提高蠕虫的掉头频率（p  <0.01），延长其寿命，增强包括GR在内的抗氧化系统63.96％（p  <0.05），GSH -Px升高71.16％（p  <0.01），SOD升高78.65％（p  <0.01），CAT 升高98.52％（p <0.01），GSH升高28.12％（p  <0.05），降低MDA降低39.29％（p  <0.01）。qRT-PCR结果表明，AAPs-H可以上调skn-1，sod-1，sod-2，sod-3的mRNA表达水平。当用0.4 mg / mL的浓度处理时，野生型秀丽隐杆线虫中的Sir-2.1和> 1.6倍（p  <0.05或p  <0.01）。我们的研究提供了证据，表明AAPs-H可改善抗氧化防御系统，并通过上调氧化应激相关基因来预防秀丽隐杆线虫的应激损伤。