A novel seedbed-like scaffold was firstly fabricated by the “frozen sectioning” processing method using Flammulina velutipes as a raw material. The Flammulina velutipes polysaccharides scaffold is composed of a natural structure imitating the “ground” (connected and aligned hollow tubes with porous walls). Meanwhile, its biologically active components include polysaccharides and proteins, mimicking the “plant nutrition” in the seedbed. To further optimize the ground and nutrition components, Flammulina velutipes polysaccharides–derived scaffolds (FPDSs) were fabricated via the treatment of original Flammulina velutipes polysaccharides scaffold (labeled FPS) by NaOH, cysteine (labeled as FPS/NaOH, FPS/Cys, respectively). FPDSs were characterized by SEM, FTIR, XRD, water absorption and retention, and mechanical evaluations. From the results, FPS/NaOH and FPS/Cys lost the characteristic big tubes of original strips and had higher water absorption capacities comparing to FPS. Simultaneously, FPS/NaOH had better ductility, FPS/Cys had showed increased stiffness. Biological activities of FPDSs were tested against different types of bacteria exhibiting excellent anti-bacterial activity, and FPS/NaOH and FPS/Cys had dramatically higher anti-bacterial activity than FPS. The cytocompatibility of FPDSs was evaluated utilizing mouse fibroblast cell line (L929), and all FPDSs showed good cytocompatibility. The FPDSs were further applied to a rat full-thickness skin wound model, and they all exhibited obviously accelerated re-epithelialization, among which FPS/NaOH showed the greatest efficiency. FPS/NaOH could shorten the wound-healing process as evidenced by dynamic alterations of the expression levels of specific stagewise markers in the healing areas. Similarly, FPS/NaOH can efficiently induce hair follicle regeneration in the healing skin tissues. In summary, FPDSs exhibit potential functions as seedbeds to promote the regeneration of the “seed” including hair follicles and injured skin, opening a new avenue for wound healing.

首先以金针菇为原料，通过“冷冻切片”加工方法制备了新型的苗床状支架。的金针菇多糖骨架是由一种自然结构模仿“接地”（连接和对准具有多孔壁的中空管）的。同时，其生物活性成分包括多糖和蛋白质，模仿了苗床中的“植物营养”。为了进一步优化地面和营养成分，金针菇通过用NaOH，半胱氨酸（分别标记为FPS / NaOH，FPS / Cys）处理原始金针菇多糖支架（标记为FPS）来制备多糖衍生的支架（FPDS）。通过SEM，FTIR，XRD，吸水率和保留率以及机械评估来表征FPDS。结果表明，与FPS相比，FPS / NaOH和FPS / Cys失去了原始条带的特征性大管，并且具有更高的吸水能力。同时，FPS / NaOH具有更好的延展性，FPS / Cys具有更高的刚度。测试了FPDS对不同类型细菌的生物活性，这些细菌均表现出出色的抗菌活性，FPS / NaOH和FPS / Cys的抗菌活性远高于FPS。使用小鼠成纤维细胞系（L929）评估FPDS的细胞相容性，并且所有FPDS都显示出良好的细胞相容性。FPDSs进一步应用于大鼠全层皮肤创面模型，它们均表现出明显的加速上皮再生，其中FPS / NaOH表现出最大的效率。FPS / NaOH可以缩短伤口愈合过程，这可以通过愈合区域特定阶段性标志物表达水平的动态变化来证明。同样，FPS / NaOH可以有效地在愈合的皮肤组织中诱导毛囊再生。总之，FPDS具有潜在的功能，可以作为种子床来促进“种子”（包括毛囊和受伤的皮肤）的再生，从而为伤口愈合开辟了新途径。它们均表现出明显的加速上皮再生，其中FPS / NaOH表现出最大的效率。FPS / NaOH可以缩短伤口愈合过程，这可以通过愈合区域特定阶段性标志物表达水平的动态变化来证明。同样，FPS / NaOH可以在愈合的皮肤组织中有效诱导毛囊再生。总之，FPDS具有潜在的功能，可以作为种子床来促进“种子”（包括毛囊和受伤的皮肤）的再生，从而为伤口愈合开辟了新途径。它们均表现出明显的加速上皮再生，其中FPS / NaOH表现出最大的效率。FPS / NaOH可以缩短伤口愈合过程，这可以通过愈合区域特定阶段性标志物表达水平的动态变化来证明。同样，FPS / NaOH可以在愈合的皮肤组织中有效诱导毛囊再生。总之，FPDS具有潜在的功能，可以作为种子床来促进“种子”（包括毛囊和受伤的皮肤）的再生，从而为伤口愈合开辟了新途径。FPS / NaOH可以有效地在愈合的皮肤组织中诱导毛囊再生。总之，FPDS具有潜在的功能，可以作为种子床来促进“种子”（包括毛囊和受伤的皮肤）的再生，从而为伤口愈合开辟了新途径。FPS / NaOH可以有效地在愈合的皮肤组织中诱导毛囊再生。总之，FPDS具有潜在的功能，可以作为种子床来促进“种子”（包括毛囊和受伤的皮肤）的再生，从而为伤口愈合开辟了新途径。