In the present study, an analytical methodology based on natural deep eutectic solvents (NADESs) and ultrasound-assisted extraction (UAE) is demonstrated as an environmentally friendly protocol for the isolation of valuable phenolic compounds from inflorescences of Helichrysum arenarium L. For this purpose, 11 different NADESs containing choline chloride and selected hydrogen bond donors, including organic acids, polyols, sugars and an amide, were tailored and evaluated based on the content of individual compounds determined by HPLC-PDA-MSⁿ. Choline chloride-lactic acid based NADES (NADES 1) showed the most promising extraction efficiencies for flavonoids (15.34 ± 0.10 mg g⁻¹) and phenolic acids (5.10 ± 0.12 mg g⁻¹) as the efficiencies were comparable to those obtained with 80 % methanol (15.60 ± 0.09 mg g⁻¹ and 5.32 ± 0.18 mg g⁻¹, respectively) and up to 54 % higher compared to water (9.94 ± 0.13 mg g⁻¹ and 5.47 ± 0.22 mg g⁻¹). Furthermore, three essential parameters of the proposed NADES 1 methodology (UAE time, water content in NADES, and NADES hydrogen donor/acceptor molar ratio) were optimized by Box-Behnken experimental design. Based on the statistically evaluated results the following conditions were determined to be optimal: an UAE time of 85 min, 38 % water in NADES 1, and a molar ratio of choline chloride:lactic acid of 1:4. The developed approach allows easy transfer of the proposed methodology to large-scale extraction, enabling clean production with minimal environmental impact.

在本研究中，基于天然深共熔溶剂（NADES）和超声辅助萃取（UAE）的分析方法被证明是一种从蜡菊蜡菊花序中分离出有价值的酚类化合物的环境友好方案。为此，根据由HPLC-PDA-MS ⁿ测定的单个化合物的含量，对11种不同的含有胆碱氯化物和选定的氢键供体的NADES（包括有机酸，多元醇，糖和酰胺）进行了定制和评估。基于胆碱氯化物-乳酸的NADES（NADES 1）对类黄酮（15.34±0.10 mg g ^-1）和酚酸（5.10±0.12 mg g ^-1）表现出最有前途的提取效率），因为其效率可与使用80％甲醇（分别为15.60±0.09 mg g ^-1和5.32±0.18 mg g ^-1）获得的效率相媲美，并且与水相比（9.94±0.13 mg g ^-1和0.94±0.13 mg g ^-1） 5.47±0.22毫克g ^-1）。此外，通过Box-Behnken实验设计优化了拟议的NADES 1方法的三个基本参数（UAE时间，NADES中的水含量以及NADES氢供体/受体摩尔比）。根据统计评估的结果，确定以下条件为最佳：UAE时间为85分钟，在NADES 1中为38％的水，氯化胆碱：乳酸的摩尔比为1：4。开发的方法可以轻松地将建议的方法转移到大规模提取中，从而实现清洁生产且对环境的影响最小。