Plant micropropagation has been proposed as a promising solution for the improvement of medicinal plants and the commercial production of phytochemicals. Tree Wormwood (Artemisia arborescens, Asteraceae) has been identified as a promising source for chamazulene production, a phytochemical compound with anti-inflammatory activity. However, the concurrent presence of β-thujone, an acute toxic agent is a significant limitation of Tree Wormwood utilization in essential oil (EO) production. Present study is aiming to develop in vitro cultivation protocols of tree wormwood and study the effects of plant growth regulators (PGRs) and culture conditions on the callus production of volatile metabolites (VM) and its equilibrium of chamazulene and β-thujone. Three treatments all containing the auxin 1-Naphthaleneacetic acid (NAA), and each one respectively the cytokines Kinetin (K), 16-Benzylaminopurine (BN), and Thidiazuron (TD) in Murashige and Skoog (MS) basal media, were applied under constant dark (D), while K and BN were also grown under light (L). Both EO and VMs, retrieved from the naturally and laboratory grown biomass were analyzed by a Gas Chromatographer-Mass Spectrometer (GC–MS) coupled with Gas Chromatographer-Flame Ionization Detector (GC-FID). The biotechnologically produced EOs presented a significantly increased yield per area and time, while callogenesis PGRs combinations and culture conditions exhibited promising results on the induction of chamazulene biosynthesis.

已经提出植物微繁殖作为改善药用植物和植物化学产品商业化生产的有前途的解决方案。树艾草（蒿木，菊科）已被确定为生产富马甲烯（一种具有抗炎活性的植物化学化合物）的有前途的来源。然而，同时存在的一种急性毒性剂β- thujone严重限制了树艾草在精油（EO）生产中的利用。目前的研究旨在开发树艾草的体外培养方案，并研究植物生长调节剂（PGR）和培养条件对挥发性代谢物（VM）愈伤组织产生及其Chamazulene和β平衡的影响。-thujone。分别在Murashige和Skoog（MS）基础培养基中使用三种处理剂，分别包含生长素1-萘乙酸（NAA）和细胞因子Kinetin（K），16-苄氨基嘌呤（BN）和Thidiazuron（TD），每种处理剂均在恒定的黑暗（D），而K和BN也在光照（L）下生长。通过气相色谱-质谱联用仪（GC-MS）和气相色谱-火焰电离检测器（GC-FID）对从自然和实验室生长的生物质中回收的EO和VM进行分析。通过生物技术生产的EOs的单位面积和时间产量均显着提高，而愈伤组织PGRs组合和培养条件在诱导马甲烯生物合成方面显示出令人鼓舞的结果。