As a specific biomarker, neuron-specific enolase (NSE) is an essential clinical indicator for diagnosing small cell lung cancer. In this paper, a sandwich-type electrochemical immunosensor was designed for the quantitative detection of NSE. AuPt nanoblock spherical nanoarchitectonics (AuPt NSNs), a bimetallic nanoparticle with a rugged morphology, were utilized as the substrate, which could enhance the electronic conduction and increase the immobilization capacity of the primary antibody (Ab₁). Moreover, through a simple hydrothermal method, Au/Cu_xO@CeO₂ was prepared as a spiny core-shell nanocube with cerium dioxide (CeO₂) and gold nanoparticles (Au NPs) loading. The combination of Cu₂O, CuO, and CeO₂ showed favorable catalytic activity toward hydrogen peroxide (H₂O₂). Furthermore, the deposition of Au NPs on the spiny surface structure enhanced the specific surface area and biocompatibility, thereby rendering it more effective for loading the second antibody (Ab₂). As the label material, the Au/Cu_xO@CeO₂ achieved signal amplification and sensitive detection with the immunosensor. Under optimal conditions, the designed immunosensor possessed a broad linear range of 50 fg mL⁻¹ to 100 ng mL⁻¹ and a limit of detection of 31.3 fg mL⁻¹, along with satisfactory performance in sensitivity, selectivity, and stability.

作为特定的生物标志物，神经元特异性烯醇化酶（NSE）是诊断小细胞肺癌的重要临床指标。本文设计了一种夹心型电化学免疫传感器，用于定量检测NSE。具有凹凸不平形态的双金属纳米颗粒AuPt纳米嵌段球形纳米结构（AuPt NSNs）被用作底物，可以增强电子传导并增加一抗（Ab ₁）的固定能力。此外，通过一种简单的水热法，制备了Au / Cu _x O @ CeO ₂为具有二氧化铈（CeO ₂）和金纳米颗粒（Au NPs）负载的多刺核壳纳米立方体。Cu ₂ O，CuO和CeO的组合_图2显示出对过氧化氢（H ₂ O ₂）的有利催化活性。此外，Au NP在多刺的表面结构上的沉积增强了比表面积和生物相容性，从而使其更有效地负载第二抗体（Ab ₂）。作为标记材料，Au / Cu _x O @ CeO ₂通过免疫传感器实现了信号放大和灵敏检测。在最佳条件下，设计的免疫传感器具有50 fg mL ^-1至100 ng mL ^-1的宽线性范围和31.3 fg mL ^-1的检测限，并在灵敏度，选择性和稳定性方面均具有令人满意的性能。