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Effect of eCG treatment on gene expression of selected matrix metalloproteinases (MMP-2, MMP-7, MMP-9, MMP-10, and MMP-13) and the tissue inhibitors of metalloproteinases (TIMP-2 and TIMP-3) in the chicken ovary
Animal Reproduction Science ( IF 2.2 ) Pub Date : 2020-11-26 , DOI: 10.1016/j.anireprosci.2020.106666
Dominika Wolak 1 , Andrzej Sechman 1 , Anna Hrabia 1
Affiliation  

Several metalloproteinases (MMPs) are present and functional in the chicken ovary and regulate the extracellular matrix (ECM) during follicle development, ovulation, atresia, and regression. The regulation of the abundance of MMPs in avian ovarian follicles, however, is largely unknown. The aim of the present study was to examine effects of equine chorionic gonadotropin (eCG) on abundance of selected MMPs and relevant tissue inhibitors of MMPs (TIMPs) in the hen ovary. The MMP-2 and MMP-9 activity was also determined. Results indicated there were effects of eCG on abundances of MMP-2, MMP-7, MMP-9, MMP-10, MMP-13, TIMP-2, and TIMP-3 mRNA transcript and/or protein relative abundances in white, yellowish, small yellow, and the largest yellow preovulatory (F3-F1) ovarian follicles. The response to eCG depended on the stage of follicle development, layer of follicular wall, and the type of MMPs or TIMPs affected by eCG. Furthermore, there was a pause in egg laying when eCG was administered and there were morphological changes in the ovary following eCG treatment that were associated with alterations in MMP-2 and MMP-9 activity. In general, the results indicate that eCG, which has primarily follicle stimulating hormone (FSH)-like bioactivities, is a negative regulator of MMP abundance and activity in the largest yellow preovulatory follicles. Results from the present study indicate the gonadotropins, especially FSH, by the regulation of transcription, translation, and/or activity of proteins of the MMP system have effects on the mechanisms that underlie ECM remodeling and cell function throughout ovarian follicle development in the chicken ovary.



中文翻译:

eCG 治疗对选定的基质金属蛋白酶(MMP-2、MMP-7、MMP-9、MMP-10 和 MMP-13)和金属蛋白酶组织抑制剂(TIMP-2 和 TIMP-3)基因表达的影响鸡卵巢

几种金属蛋白酶 (MMP) 存在于鸡卵巢中并发挥作用,它们在卵泡发育、排卵、闭锁和退化过程中调节细胞外基质 (ECM)。然而,禽卵泡中 MMP 丰度的调节在很大程度上是未知的。本研究的目的是检查马绒毛膜促性腺激素 (eCG) 对母鸡卵巢中所选 MMP 和相关 MMP 组织抑制剂 (TIMP) 丰度的影响。还测定了 MMP-2 和 MMP-9 活性。结果表明心电图对MMP-2、MMP-7、MMP-9、MMP-10、MMP-13、TIMP-2 和 TIMP-3 的丰度有影响白色、淡黄色、小黄色和最大的黄色排卵前 (F3-F1) 卵泡中的 mRNA 转录物和/或蛋白质相对丰度。对心电图的反应取决于卵泡发育阶段、卵泡壁层数以及受心电图影响的 MMP 或 TIMP 类型。此外,当给予 eCG 时产卵会暂停,并且在 eCG 治疗后卵巢出现形态学变化,这与 MMP-2 和 MMP-9 活性的改变有关。总的来说,结果表明,eCG 主要具有促卵泡激素 (FSH) 样生物活性,是最大的黄色排卵前卵泡中 MMP 丰度和活性的负调节剂。本研究结果表明促性腺激素,尤其是 FSH,通过调节转录、翻译、

更新日期:2020-12-01
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