This study was conducted to evaluate the antimicrobial potential of the antimicrobial peptides (AMP) LL-37 and human Lactoferricin (LfcinH) on the planktonic growth and biofilm formation of oral pathogenic anaerobes related to caries and periodontitis. Multi-species bacterial suspensions of either facultative anaerobic bacteria (FAB: Streptococcus mutans, Streptococcus sanguinis, Actinomyces naeslundii) or obligate anaerobic bacteria (OAB: Veillonella parvula, Parvimonas micra, Fusobacterium nucleatum) were incubated with different concentrations of AMP solutions for 8 h. Planktonic growth was registered with an ATP-based cell viability assay for FAB and via plate counting for OAB. Biofilms were grown on ZrO₂ discs for 4 days in a mixture of the multi-species bacterial suspensions and AMP solutions. Biofilm mass was quantified using a microtiter plate biofilm assay with crystal violet staining. An overall planktonic growth inhibition and biofilm mass reduction of FAB and OAB was registered for LL-37 and LfcinH. Significant inhibitory threshold concentrations of LL-37 were observed in all experiments (p < 0.0001). No significant threshold was observed for LfcinH. Biofilm mass of OAB was barely reduced by LfcinH. The complete mechanisms of the AMPs are not fully understood yet. While LL-37 shows promising features as potential therapeutic for biofilm-associated oral diseases, LfcinH seems unsuitable for this particular indication. For clinical AMP use, further investigations will be necessary.

本研究旨在评估抗菌肽 (AMP) LL-37 和人乳铁蛋白 (LfcinH) 对与龋齿和牙周炎相关的口腔致病性厌氧菌的浮游生长和生物膜形成的抗菌潜力。多品种或者兼性厌氧细菌的细菌悬浮液（FAB：变形链球菌，血链球菌，放线菌naeslundii）或专性厌氧菌（OAB：小韦荣菌，Parvimonas MICRA，具核梭杆菌) 与不同浓度的 AMP 溶液孵育 8 小时。使用基于 ATP 的 FAB 细胞活力测定和 OAB 平板计数记录浮游生长。生物膜在 ZrO ₂上生长在多物种细菌悬浮液和 AMP 溶液的混合物中放置 4 天。使用具有结晶紫染色的微量滴定板生物膜测定来量化生物膜质量。对于 LL-37 和 LfcinH，FAB 和 OAB 的整体浮游生长抑制和生物膜质量减少被登记。在所有实验中都观察到 LL-37 的显着抑制阈值浓度 (p < 0.0001)。没有观察到 LfcinH 的显着阈值。LfcinH 几乎没有减少 OAB 的生物膜质量。AMP 的完整机制尚未完全了解。虽然 LL-37 作为生物膜相关口腔疾病的潜在治疗剂显示出有希望的特性，但 LfcinH 似乎不适合这种特殊的适应症。对于临床 AMP 使用，需要进一步研究。