The objective of the study was to investigate the bactericidal effectiveness of atmospheric pressure nonthermal plasma (NTP) on biofilms of Staphylococcus aureus and Escherichia coli formed on the surface of surgical sutures. Tests were carried out for polyamide nonabsorbable monofilament sutures and mid-term absorbable synthetic monofilament sutures made of glyconate. Dielectric barrier discharge reactor providing atmospheric air plasma and constant discharge power density of 70 mW/cm 2 was used in the experiments. The colonization/decontamination efficacy was quantified by colony-counting assay. It was shown that decontamination efficacy of the studied surgical sutures was time-dependent i.e. the effect was more visible for longer exposure times ranging from 60 to 360 s. It was proposed to combine plasma treatment with washing the biofilms with a phosphate buffered saline to to allow the plasma to interact with cells in the deeper layers of bacterial biofilm. The application of the original method of treatment of biofilms was more effective than the separate plasma treatment. As a result, the lethal effect was most often achieved after 300 s regardless of the type of suture and biofilm. The results of the comparative studies on the decontamination efficiency of polymeric surgical suture surface by disinfectants and NTP technique have shown unequivocally that plasma treatment is the most effective way to deactivate bacterial cells and ensures a lethal effect for most of the tested cases.

中文翻译：

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使用非热等离子体净化覆盖有细菌生物膜的聚合手术缝线

该研究的目的是研究大气压非热等离子体 (NTP) 对手术缝合线表面形成的金黄色葡萄球菌和大肠杆菌生物膜的杀菌效果。对聚酰胺不可吸收单丝缝合线和由甘氨酸盐制成的中期可吸收合成单丝缝合线进行了测试。在实验中使用提供大气等离子体和70mW/cm 2 的恒定放电功率密度的介质阻挡放电反应器。通过菌落计数测定来量化定殖/净化功效。结果表明，所研究的手术缝合线的去污功效与时间有关，即对于 60 到 360 秒的较长暴露时间，效果更明显。有人提议将等离子体处理与用磷酸盐缓冲盐水清洗生物膜相结合，以允许等离子体与细菌生物膜更深层中的细胞相互作用。应用原始的生物膜处理方法比单独的等离子体处理更有效。因此，无论缝合线和生物膜的类型如何，最常在 300 秒后达到致死效果。消毒剂和NTP技术对聚合手术缝合线表面去污效率的比较研究结果明确表明，等离子体处理是灭活细菌细胞的最有效方法，并确保对大多数测试病例具有致死作用。应用原始的生物膜处理方法比单独的等离子体处理更有效。因此，无论缝合线和生物膜的类型如何，最常在 300 秒后达到致死效果。消毒剂和NTP技术对聚合手术缝合线表面去污效率的比较研究结果明确表明，等离子体处理是灭活细菌细胞的最有效方法，并确保对大多数测试病例具有致死作用。应用原始的生物膜处理方法比单独的等离子体处理更有效。因此，无论缝合线和生物膜的类型如何，最常在 300 秒后达到致死效果。消毒剂和NTP技术对聚合手术缝合线表面去污效率的比较研究结果明确表明，等离子体处理是灭活细菌细胞的最有效方法，并确保对大多数测试病例具有致死作用。