Human embryonic stem cell-derived retinal pigment epithelial (hESC-RPE) cell transplants have served as a cell therapy for treating retinal degenerative diseases. However, how to optimize the survival and engraftment of hESC-RPE cells is a great challenge. Here, we report hESC-RPE cells that are embedded with polyelectrolytes gelatin and alginate by layer-by-layer (LbL) self-assembly technique, based on the opposite charge of alternate layers. Cells were assessed for cell survival, immunogenicity, and function in vitro and in vivo. This strategy obviously decreased the immunogenicity of hESC-RPE cells without affecting its activity. LbL-RPE cell transplants into the subretinal space of Royal College of Surgeons (RCS) rats optimized cell engraftment and decreased immunogenicity compared to untreated RPE cell transplants (immunosuppression was not used during the 21-week study). Visual-functional assay with electroretinogram recordings (ERGs) also showed higher B wave amplitudes in RCS rats with LbL-RPE cell transplants. We demonstrate that transplanted LbL-RPE cells have better viability and grafting efficiency, optimized immunogenicity, and visual function. Therefore, LbL engineering is a promising method to increase the efficacy of hESC-RPE cell transplantation.

中文翻译：

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通过hESC衍生的RPE细胞的逐层纳米涂层改善体内细胞存活和植入

人类胚胎干细胞源性视网膜色素上皮细胞（hESC-RPE）的细胞移植已作为一种治疗视网膜变性疾病的细胞疗法。然而，如何优化hESC-RPE细胞的存活和植入是巨大的挑战。在这里，我们报告了hESC-RPE细胞，这些蛋白通过交替层的相反电荷通过逐层（LbL）自组装技术嵌入了聚电解质明胶和藻酸盐。评估细胞在体外和体内的细胞存活，免疫原性和功能。该策略明显降低了hESC-RPE细胞的免疫原性而不影响其活性。与未经处理的RPE细胞移植相比，将LbL-RPE细胞移植到英国皇家外科医学院（RCS）大鼠的视网膜下空间可优化细胞植入并降低免疫原性（在21周的研究中未使用免疫抑制）。带有视网膜电图记录（ERG）的视觉功能测定法还显示，在LbL-RPE细胞移植的RCS大鼠中，B波振幅更高。我们证明，移植的LbL-RPE细胞具有更好的生存力和移植效率，优化的免疫原性和视觉功能。因此，LbL工程是提高hESC-RPE细胞移植功效的一种有前途的方法。我们证明，移植的LbL-RPE细胞具有更好的生存力和移植效率，优化的免疫原性和视觉功能。因此，LbL工程是提高hESC-RPE细胞移植功效的一种有前途的方法。我们证明，移植的LbL-RPE细胞具有更好的生存力和移植效率，优化的免疫原性和视觉功能。因此，LbL工程是提高hESC-RPE细胞移植功效的一种有前途的方法。