Tissue adhesives have been widely used in surgery to treat pulmonary air leaks. However, conventional adhesives have poor interfacial strength under wet conditions. To overcome this clinical problem, we modified Alaska pollock-derived gelatin to include decanyl (C10) groups (C10-ApGltn) and used electrospinning to create a tissue-adhesive fiber sheet (AdFS). C10-AdFS showed higher burst strength when adhering to porcine pleura compared with a sheet of original ApGltn (Org-ApGltn). Hematoxylin–eosin-stained sections after burst experiments reveal that a dense C10-AdFS layer remained on the surface of the porcine pleura. The effect of the degree of C10 modification of ApGltn on the burst strength was evaluated. ApGltn with a C10 modification ratio of 13 mol% amino groups (13C10-AdFS) exhibited the highest burst strength. Furthermore, from ex vivo experiments with extracted rat lung, 13C10-AdFS exhibited a higher burst strength (41 cm H₂O) than Org-AdFS. The decanyl groups in 13C10-AdFS interacted with the hydrophobic proteins and the lipid bilayers of the cells, resulting in the high interfacial strength between 13C10-AdFS and the pleura. Moreover, 13C10-AdFS samples implanted subcutaneously in the backs of rats were completely degraded within 21 days without any severe inflammation. These results show that 13C10-AdFS is a promising adhesive material for the treatment of pulmonary air leaks.

中文翻译：

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使用基于用癸基改性的阿拉斯加狭鳕明胶的可生物降解组织粘附纤维片预防肺漏气

组织粘合剂已广泛用于手术治疗肺漏气。然而，传统粘合剂在潮湿条件下的界面强度较差。为了克服这个临床问题，我们对阿拉斯加狭鳕衍生的明胶进行了改良，使其包含癸基 (C10) 基团 (C10-ApGltn)，并使用静电纺丝来制造组织粘合纤维片 (AdFS)。与原始 ApGltn (Org-ApGltn) 片材相比，C10-AdFS 在粘附于猪胸膜时表现出更高的爆裂强度。爆裂实验后苏木精-伊红染色切片显示，猪胸膜表面保留有致密的 C10-AdFS 层。评估了 ApGltn 的 C10 改性程度对爆裂强度的影响。具有 13 mol% 氨基的 C10 修饰率的 ApGltn (13C10-AdFS) 表现出最高的爆发强度。此外，从用提取的大鼠肺进行离体实验，13C10-AdFS 表现出比 Org-AdFS 更高的爆发强度 (41 cm H ₂ O)。13C10-AdFS 中的癸基与疏水蛋白和细胞的脂质双层相互作用，导致 13C10-AdFS 和胸膜之间的高界面强度。此外，在大鼠背部皮下植入的 13C10-AdFS 样本在 21 天内完全降解，没有任何严重的炎症。这些结果表明，13C10-AdFS 是一种很有前途的用于治疗肺漏气的粘合剂材料。