SummaryThe present study investigated the effects of c-type natriuretic peptide (CNP) on the development of murine preantral follicles during in vitro growth (IVG). Preantral follicles isolated from ovaries of Kunming mice were cultured in vitro. In the culture system, CNP was supplemented in the experimental groups and omitted in the control groups. In Experiment 1, CNP was only supplemented at the early stage and follicle development was evaluated. In Experiments 2 and 3, CNP was supplemented during the whole period of in vitro culture. In Experiment 2, follicle development and oocyte maturity were evaluated. In Experiment 3, follicle development and embryo cleavage after in vitro fertilization (IVF) were assessed. The results showed that in the control groups in all three experiments, granulosa cells migrated from within the follicle and the follicles could not reach the antral stage. In the experimental groups in all three experiments, no migration of granulosa cells was observed and follicle development was assessed as attaining the antral stage, which was significantly superior to that of the control group (P < 0.0001). Oocyte meiotic arrest was effectively maintained, hence giving good developmental competence. In conclusion, CNP supplementation in the culture system during IVG benefited the development of murine preantral follicles.

中文翻译：

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体外生长期补充c型利钠肽有利于小鼠窦前卵泡发育

摘要本研究调查了 c 型利钠肽 (CNP) 对小鼠胃窦前卵泡发育的影响。体外生长（IVG）。培养昆明小鼠卵巢腔前卵泡体外. 在培养体系中，实验组补充CNP，对照组省略。在实验1中，仅在早期补充CNP并评估卵泡发育。在实验 2 和 3 中，CNP 是在整个过程中补充的。体外文化。在实验 2 中，评估了卵泡发育和卵母细胞成熟度。在实验 3 中，卵泡发育和胚胎卵裂后体外评估受精（IVF）。结果表明，三个实验的对照组中，颗粒细胞均从毛囊内迁移，毛囊无法到达窦期。在所有三个实验中，实验组均未观察到颗粒细胞的迁移，且卵泡发育被评估为达到窦期，明显优于对照组（磷< 0.0001)。卵母细胞减数分裂停滞得到有效维持，因此具有良好的发育能力。总之，IVG 期间在培养系统中补充 CNP 有利于小鼠窦前卵泡的发育。